Quantitative analysis of miRNAs using SplintR ligase-mediated ligation of complementary-pairing probes enhanced by RNase H (SPLICER)-qPCR
Here, a method using SplintR ligase-mediated ligation of complementary-pairing probes enhanced by RNase H (SPLICER) for miRNAs quantification was established. The strategy has two steps: (1) ligation of two DNA probes specifically hybridize to target miRNA and (2) qPCR amplifying the ligated probe....
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Main Authors: | Xinshu Qin (Author), Xingyu Wang (Author), Ke Xu (Author), Yi Zhang (Author), Hongye Tian (Author), Yinglei Li (Author), Bangran Qi (Author), Xingbin Yang (Author) |
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Format: | Book |
Published: |
Elsevier,
2023-03-01T00:00:00Z.
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Online Access: | Connect to this object online. |
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