Cellular response of human apical papilla cells to calcium hydroxide and tricalcium silicate-based cements
Abstract Background This study aimed to evaluate the biological response of human apical papilla cells to different calcium hydroxide formulations and three tricalcium silicate-based materials. Methods Primary cells were obtained from explants of young immature premolars. 20,000 cells adhered for 24...
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2021-03-01T00:00:00Z.
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| LEADER | 00000 am a22000003u 4500 | ||
|---|---|---|---|
| 001 | doaj_39cfac0db8d7496d919cc870c1493d57 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Mauricio Garrido |e author |
| 700 | 1 | 0 | |a Diego Morales |e author |
| 700 | 1 | 0 | |a María Paz Saldías |e author |
| 700 | 1 | 0 | |a Christian Fernández |e author |
| 700 | 1 | 0 | |a Veronica Villalobos |e author |
| 700 | 1 | 0 | |a Oscar Cerda |e author |
| 700 | 1 | 0 | |a Mónica Cáceres |e author |
| 245 | 0 | 0 | |a Cellular response of human apical papilla cells to calcium hydroxide and tricalcium silicate-based cements |
| 260 | |b BMC, |c 2021-03-01T00:00:00Z. | ||
| 500 | |a 10.1186/s12903-021-01467-6 | ||
| 500 | |a 1472-6831 | ||
| 520 | |a Abstract Background This study aimed to evaluate the biological response of human apical papilla cells to different calcium hydroxide formulations and three tricalcium silicate-based materials. Methods Primary cells were obtained from explants of young immature premolars. 20,000 cells adhered for 24 h over discs of Biodentine™, ProRoot®MTA, BioRoot®RCS and calcium hydroxide mixed either with sodium chloride 0.9%w/v or polyethylene glycol and UltraCal® were used to evaluate cell adhesion by scanning electron microscopy and cell viability by MTT assay. Results Cells adhered to ProRoot®MTA showed an increase of F-actin like protrusions, suggesting bioactivity. Cells adhered to UltraCal® show protrusion such as filopodia. On the contrary, cells adhered to BioRoot®RCS showed no signs of any cellular protrusion. Regarding viability between the materials, we found a higher percentage of viability in cells cultured over discs of Biodentine™ and ProRoot®MTA. Conclusion ProRoot®MTA and Biodentine™ exhibit a better cellular response of human apical papilla cells in vitro conditions compared to BioRoot® and calcium hydroxide diluted in sodium chloride. | ||
| 546 | |a EN | ||
| 690 | |a Bioceramics | ||
| 690 | |a Apical papilla | ||
| 690 | |a Calcium hydroxide | ||
| 690 | |a Dentistry | ||
| 690 | |a RK1-715 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n BMC Oral Health, Vol 21, Iss 1, Pp 1-8 (2021) | |
| 787 | 0 | |n https://doi.org/10.1186/s12903-021-01467-6 | |
| 787 | 0 | |n https://doaj.org/toc/1472-6831 | |
| 856 | 4 | 1 | |u https://doaj.org/article/39cfac0db8d7496d919cc870c1493d57 |z Connect to this object online. |