The dissemination of ST80-SCC<it>mec</it>-IV community-associated methicillin resistant <it>Staphylococcus aureus </it>clone in Kuwait hospitals
<p>Abstract</p> <p>Background</p> <p>Community-associated methicillin-resistant <it>Staphylococcus aureus </it>(CA-MRSA) is a global healthcare problem. The purpose of this study was to characterize CA-MRSA clones and their distribution in Kuwait hospitals.&...
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| Format: | Book |
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BMC,
2010-11-01T00:00:00Z.
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| Summary: | <p>Abstract</p> <p>Background</p> <p>Community-associated methicillin-resistant <it>Staphylococcus aureus </it>(CA-MRSA) is a global healthcare problem. The purpose of this study was to characterize CA-MRSA clones and their distribution in Kuwait hospitals.</p> <p>Methods</p> <p>In total, 135 CA-MRSA isolates, carrying the SCCmec IV or V genetic elements, isolated in eight hospitals were characterized using antibiogram, pulsed-field gel electrophoresis, multilocus sequence typing, and carriage of genes for Panton-Valentine Leukocidin (PVL), capsular polysaccharides types (cap) 5 and 8, accessory genes regulators (agr), Staphylococcal enterotoxins (SE) and toxic shock syndrome toxin 1 (<it>tst</it>).</p> <p>Results</p> <p>They were susceptible to vancomycin, teicoplanin and linezolid but resistant to kanamycin (62%), fusidic acid (42.2%), tetracycline (39.3%), erythromycin and clindamycin (21.5%), gentamicin (5.9%), streptomycin (6.7%), trimethoprim (5.9%), mupirocin (6.6%) and cadmium acetate (82.2%). They consisted of 10 pulsotypes with the majority belonging to PFGE type I (51.1%), type II (22.2%), type IV (13.3%) and type III (3.7%). They belonged to 10 sequence types (ST) comprising ST80 (51.1%), ST30 (22.2%), ST5 (14.1%), ST1 (4.45), ST6 (3.7%), ST88 (1.5%), ST834 (1.5%), ST8 (0.7%), ST46 (0.7%) and ST950 (0.7%). Genes for PVL, cap 8, cap 5 and agr III, agr I and agr II were detected in 61.5%, 77.3%, 20.7% and 62.2%, 17% and 8.1% of the isolates respectively. Nine (6.7%) isolates contained <it>tst </it>while 103 isolates were positive for SE genes with <it>sei </it>(63.0%), <it>seg </it>(41.5%) and <it>sed </it>(29.6%) as the common SE genes.</p> <p>Conclusions</p> <p>ST80-SCC<it>mec</it>IV was the most common CA-MRSA clone in Kuwait hospitals presenting new challenges for infection control.</p> |
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| Item Description: | 10.1186/1476-0711-9-31 1476-0711 |