The Effects of Seleno-Methionine in Cadmium-Challenged Human Primary Chondrocytes

• Main Authors: Valentina Urzì Brancati (Author), Federica Aliquò (Author), José Freni (Author), Alice Pantano (Author), Erika Galipò (Author), Domenico Puzzolo (Author), Letteria Minutoli (Author), Herbert Ryan Marini (Author), Giuseppe Maurizio Campo (Author), Angela D'Ascola (Author)
• Format: Book
• Published: MDPI AG, 2024-07-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 10.3390/ph17070936; 1424-8247
• Keywords: apoptosis; cadmium; chondrocyte; potentially toxic element; seleno-methionine; Medicine; R; Pharmacy and materia medica; RS1-441
• Source: Pharmaceuticals, Vol 17, Iss 7, p 936 (2024)

Cadmium (Cd) is a potentially toxic element able to interfere with cellular functions and lead to disease or even death. Cd accumulation has been demonstrated in cartilage, where it can induce damage in joints. The aim of this study was to evaluate the effect of CdCl₂ on primary cultures of human chondrocytes and the possible protective effect of seleno-methionine (Se-Met). Human primary articular chondrocytes were cultured and treated as follows: control groups, cells challenged with 7.5 μM and 10 μM CdCl₂ alone, and cells pretreated with 10 and 20 μM Se-Met and then challenged with 7.5 μM and 10 μM CdCl₂. Twenty-four hours after incubation, cell viability, histological evaluation with hematoxylin-eosin stain, and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay were performed. Furthermore, reverse transcription-PCR was carried out to evaluate mRNA levels of <i>BAX</i>, <i>BAK1</i>, <i>CASP-3</i>, and <i>CASP-9</i>. After CdCl₂ challenge at both doses, a reduced cell viability and an overexpression of <i>BAX</i>, <i>BAK1</i>, <i>CASP-3</i>, and <i>CASP-9</i> genes, as well as a high number of TUNEL-positive cells, were demonstrated, all parameters becoming higher as the dose of CdCl₂ was increased. The pretreatment with Se-Met lowered the expression of all considered genes, improved cell viability and morphological changes, and reduced the number of TUNEL-positive cells. It was concluded that Se-Met plays a protective role against CdCl₂-induced structural and functional changes in chondrocytes in vitro, as it improved cell viability and showed a positive role in the context of the apoptotic pathways. It is therefore suggested that a translational, multifaceted approach, with plant-based diets, bioactive functional foods, nutraceuticals, micronutrients, and drugs, is possibly advisable in situations of environmental pollution caused by potentially toxic elements.