miRNA-26a-5p Accelerates Healing via Downregulation of PTEN in Fracture Patients with Traumatic Brain Injury

• Main Authors: Yuan Xiong (Author), Faqi Cao (Author), Liangcong Hu (Author), Chenchen Yan (Author), Lang Chen (Author), Adriana C. Panayi (Author), Yun Sun (Author), Wu Zhou (Author), Peng Zhang (Author), Qipeng Wu (Author), Hang Xue (Author), Mengfei Liu (Author), Yi Liu (Author), Jing Liu (Author), Abudula Abududilibaier (Author), Bobin Mi (Author), Guohui Liu (Author)
• Format: Book
• Published: Elsevier, 2019-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Patients who sustain a traumatic brain injury (TBI) are known to have a significantly quicker fracture healing time than patients with isolated fractures, but the underlying mechanism has yet to be identified. In this study, we found that the upregulation of miRNA-26a-5p induced by TBI correlated with a decrease in phosphatase and tensin homolog (PTEN) in callus formation. In vitro, overexpressing miRNA-26a-5p inhibited PTEN expression and accelerated osteoblast differentiation, whereas silencing of miRNA-26a-5p inhibited osteoblast activity. Reduction of PTEN facilitated osteoblast differentiation via the PI3K/AKT signaling pathway. Through luciferase assays, we found evidence that PTEN is a miRNA-26a-5p target gene that negatively regulates the differentiation of osteoblasts. Moreover, the present study confirmed that preinjection of agomiR-26a-5p leads to increased bone formation. Collectively, these results indicate that miRNA-26a-5p overexpression may be a key factor governing the improved fracture healing observed in TBI patients after the downregulation of PTEN and PI3K/AKT signaling. Upregulation of miRNA-26a-5p may therefore be a promising therapeutic strategy for promoting fracture healing. Keywords: traumatic brain injury, fracture, miRNA, PTEN

Item Description

2162-2531
10.1016/j.omtn.2019.06.001