miRNA-26a-5p Accelerates Healing via Downregulation of PTEN in Fracture Patients with Traumatic Brain Injury
Patients who sustain a traumatic brain injury (TBI) are known to have a significantly quicker fracture healing time than patients with isolated fractures, but the underlying mechanism has yet to be identified. In this study, we found that the upregulation of miRNA-26a-5p induced by TBI correlated wi...
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| Main Authors: | , , , , , , , , , , , , , , , , |
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Elsevier,
2019-09-01T00:00:00Z.
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| 100 | 1 | 0 | |a Yuan Xiong |e author |
| 700 | 1 | 0 | |a Faqi Cao |e author |
| 700 | 1 | 0 | |a Liangcong Hu |e author |
| 700 | 1 | 0 | |a Chenchen Yan |e author |
| 700 | 1 | 0 | |a Lang Chen |e author |
| 700 | 1 | 0 | |a Adriana C. Panayi |e author |
| 700 | 1 | 0 | |a Yun Sun |e author |
| 700 | 1 | 0 | |a Wu Zhou |e author |
| 700 | 1 | 0 | |a Peng Zhang |e author |
| 700 | 1 | 0 | |a Qipeng Wu |e author |
| 700 | 1 | 0 | |a Hang Xue |e author |
| 700 | 1 | 0 | |a Mengfei Liu |e author |
| 700 | 1 | 0 | |a Yi Liu |e author |
| 700 | 1 | 0 | |a Jing Liu |e author |
| 700 | 1 | 0 | |a Abudula Abududilibaier |e author |
| 700 | 1 | 0 | |a Bobin Mi |e author |
| 700 | 1 | 0 | |a Guohui Liu |e author |
| 245 | 0 | 0 | |a miRNA-26a-5p Accelerates Healing via Downregulation of PTEN in Fracture Patients with Traumatic Brain Injury |
| 260 | |b Elsevier, |c 2019-09-01T00:00:00Z. | ||
| 500 | |a 2162-2531 | ||
| 500 | |a 10.1016/j.omtn.2019.06.001 | ||
| 520 | |a Patients who sustain a traumatic brain injury (TBI) are known to have a significantly quicker fracture healing time than patients with isolated fractures, but the underlying mechanism has yet to be identified. In this study, we found that the upregulation of miRNA-26a-5p induced by TBI correlated with a decrease in phosphatase and tensin homolog (PTEN) in callus formation. In vitro, overexpressing miRNA-26a-5p inhibited PTEN expression and accelerated osteoblast differentiation, whereas silencing of miRNA-26a-5p inhibited osteoblast activity. Reduction of PTEN facilitated osteoblast differentiation via the PI3K/AKT signaling pathway. Through luciferase assays, we found evidence that PTEN is a miRNA-26a-5p target gene that negatively regulates the differentiation of osteoblasts. Moreover, the present study confirmed that preinjection of agomiR-26a-5p leads to increased bone formation. Collectively, these results indicate that miRNA-26a-5p overexpression may be a key factor governing the improved fracture healing observed in TBI patients after the downregulation of PTEN and PI3K/AKT signaling. Upregulation of miRNA-26a-5p may therefore be a promising therapeutic strategy for promoting fracture healing. Keywords: traumatic brain injury, fracture, miRNA, PTEN | ||
| 546 | |a EN | ||
| 690 | |a Therapeutics. Pharmacology | ||
| 690 | |a RM1-950 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Molecular Therapy: Nucleic Acids, Vol 17, Iss , Pp 223-234 (2019) | |
| 787 | 0 | |n http://www.sciencedirect.com/science/article/pii/S2162253119301593 | |
| 787 | 0 | |n https://doaj.org/toc/2162-2531 | |
| 856 | 4 | 1 | |u https://doaj.org/article/3d2fbc5db24f43f9ace62f9ca82972e6 |z Connect to this object online. |