Induction of Apoptosis and Growth-Inhibition by Thymoquinone in ACHN and GP-293 Cell Lines in Comparable with Cis-Platinum

• Main Authors: Samira Shahraki (Author), Reza Mohebbati (Author), Mohammad Naser Shafei (Author), Mahmoud Mahmoudi (Author), Sara Hosseinian (Author), Soghra Parhizgar (Author), Zohreh Naji Ebrahimi Yazd (Author), Nazanin Entezari Heravi (Author), Reza Nejad Shahrokh Abadi (Author), Abolfazl Khajavirad (Author)
• Format: Book
• Published: Korean Pharmacopuncture Institute, 2019-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 10.3831/KPI.2019.22.024; 2093-6966; 2234-6856
• Keywords: thymoquinone; gp-293 cell line; achn cell line; cis-platinum; cytotoxicity; apoptosis; Medicine; R; Miscellaneous systems and treatments; RZ409.7-999; Therapeutics. Pharmacology; RM1-950
• Source: Journal of Pharmacopuncture, Vol 22, Iss 3, Pp 176-183 (2019)

Objective:In the current work, we investigated the cytotoxic and apoptotic effects of Thymoquinone (TQ), an active compound of Nigella sativa (N. sativa) and Cis-platinum, on normal renal epithelial (GP-293) and human renal adenocarcinoma cell lines (ACHN).Methods:GP-293 and ACHN cell lines were cultured in Dulbecco's modified Eagle's medium (DMEM) with 10% Fetal bovine serum (FBS) and 1% penicillin plus streptomycin antibiotic. The MTT assay was used for cellular viability assessment. Viability of cells was observed using inverted light microscope 24, 48 and 72 h after exposure of the cells to various concentrations of TQ (1, 2.5, 5, 10, 50 and 100 µg/ml) and Cis-platinum (0.5, 1, 1.5, 2, 3, 6 and 12.5 µg/ml). Moreover, apoptosis was analyzed with a flow-cytometry method. The untreated cells were considered as control group.Results:Morphological changes such as reduced cell number and increased intercellular distance and reduced cell viability in ACHN and GP-293cell lines were observed in both TQ and Cis-platinum groups; however, Cis-platinum had greater effect on ACHN cell line than GP-293 cell line. In addition, GP-293 cell line was more sensitive to TQ compared to ACHN cell line. Furthermore, TQ and Cis-platinum had apoptotic effects on both ACHN and GP-293 cell lines.Conclusion:Our findings demonstrated that TQ and Cis-platinum had cytotoxic and apoptotic effects on both cell lines, However, GP-293 cell line was more sensitive to TQ. Additionally, Cis-platinum was more effective on ACHN cell line than on GP-293 cell line.