Bioluminescence Imaging and ICP-MS Associated with SPION as a Tool for Hematopoietic Stem and Progenitor Cells Homing and Engraftment Evaluation
Bone marrow transplantation is a treatment for a variety of hematological and non-hematological diseases. For the transplant success, it is mandatory to have a thriving engraftment of transplanted cells, which directly depends on their homing. The present study proposes an alternative method to eval...
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MDPI AG,
2023-03-01T00:00:00Z.
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| LEADER | 00000 am a22000003u 4500 | ||
|---|---|---|---|
| 001 | doaj_3ea1f1836a1245eeb784ad2970fe9913 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Murilo M. Garrigós |e author |
| 700 | 1 | 0 | |a Fernando A. Oliveira |e author |
| 700 | 1 | 0 | |a Mariana P. Nucci |e author |
| 700 | 1 | 0 | |a Javier B. Mamani |e author |
| 700 | 1 | 0 | |a Olívia F. M. Dias |e author |
| 700 | 1 | 0 | |a Gabriel N. A. Rego |e author |
| 700 | 1 | 0 | |a Mara S. Junqueira |e author |
| 700 | 1 | 0 | |a Cícero J. S. Costa |e author |
| 700 | 1 | 0 | |a Lucas R. R. Silva |e author |
| 700 | 1 | 0 | |a Arielly H. Alves |e author |
| 700 | 1 | 0 | |a Nicole M. E. Valle |e author |
| 700 | 1 | 0 | |a Luciana Marti |e author |
| 700 | 1 | 0 | |a Lionel F. Gamarra |e author |
| 245 | 0 | 0 | |a Bioluminescence Imaging and ICP-MS Associated with SPION as a Tool for Hematopoietic Stem and Progenitor Cells Homing and Engraftment Evaluation |
| 260 | |b MDPI AG, |c 2023-03-01T00:00:00Z. | ||
| 500 | |a 10.3390/pharmaceutics15030828 | ||
| 500 | |a 1999-4923 | ||
| 520 | |a Bone marrow transplantation is a treatment for a variety of hematological and non-hematological diseases. For the transplant success, it is mandatory to have a thriving engraftment of transplanted cells, which directly depends on their homing. The present study proposes an alternative method to evaluate the homing and engraftment of hematopoietic stem cells using bioluminescence imaging and inductively coupled plasma mass spectrometry (ICP-MS) associated with superparamagnetic iron oxide nanoparticles. We have identified an enriched population of hematopoietic stem cells in the bone marrow following the administration of Fluorouracil (5-FU). Lately, the cell labeling with nanoparticles displayed the greatest internalization status when treated with 30 µg Fe/mL. The quantification by ICP-MS evaluate the stem cells homing by identifying 3.95 ± 0.37 µg Fe/mL in the control and 6.61 ± 0.84 µg Fe/mL in the bone marrow of transplanted animals. In addition, 2.14 ± 0.66 mg Fe/g in the spleen of the control group and 2.17 ± 0.59 mg Fe/g in the spleen of the experimental group was also measured. Moreover, the bioluminescence imaging provided the follow up on the hematopoietic stem cells behavior by monitoring their distribution by the bioluminescence signal. Lastly, the blood count enabled the monitoring of animal hematopoietic reconstitution and ensured the transplantation effectiveness. | ||
| 546 | |a EN | ||
| 690 | |a hematopoietic stem cells | ||
| 690 | |a bone marrow transplantation | ||
| 690 | |a bioluminescence imaging | ||
| 690 | |a ICP-MS | ||
| 690 | |a superparamagnetic iron oxide nanoparticles | ||
| 690 | |a engraftment | ||
| 690 | |a Pharmacy and materia medica | ||
| 690 | |a RS1-441 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Pharmaceutics, Vol 15, Iss 3, p 828 (2023) | |
| 787 | 0 | |n https://www.mdpi.com/1999-4923/15/3/828 | |
| 787 | 0 | |n https://doaj.org/toc/1999-4923 | |
| 856 | 4 | 1 | |u https://doaj.org/article/3ea1f1836a1245eeb784ad2970fe9913 |z Connect to this object online. |