Antioxidant Capacity and Lipid Peroxidation Product in Pulmonary Tuberculosis
Background: Severe oxidative stress has been reported in pulmonary tuberculosis (TB) patients because of increased production of reactive oxygen species (ROS) secondary to phagocyte respiratory burst, malnutrition and poor immunity but there is limited knowledge available for antioxidant status and...
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| Main Authors: | , , , |
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| Format: | Book |
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Al Ameen Medical College,
2012-07-01T00:00:00Z.
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| Summary: | Background: Severe oxidative stress has been reported in pulmonary tuberculosis (TB) patients because of increased production of reactive oxygen species (ROS) secondary to phagocyte respiratory burst, malnutrition and poor immunity but there is limited knowledge available for antioxidant status and its relation to lipid peroxidation in tuberculosis patients, particularly in developing countries. The purpose of this study was to determine the Trolox Equivalent Antioxidant Capacity (TEAC), Superoxide Dismutase (SOD) activity and Malondialdihyde (MDA), a marker of lipid peroxidation in TB patients. Methods: The study samples comprised of healthy volunteers (n=38) as control group and TB patients (n=85) undergoing anti-tuberculosis therapy (ATT). Trolox equivalent antioxidant capacity (TEAC) was determined by ABTS 2, 2ꞌ-azinobis-(3-ethylbenzeno-thiazoline-6-sulphonic diammonium salt), SOD activity by Potassium Metabisulphite-NADH-Nitroblue Tetrazolium (PMS-NADH-NBT) assay and MDA by Thiobarbituric Acid (TBA) method. Results: TEAC values (mmol/l) were decreased significantly in TB patients (0.84 ± 0.10 mmol/l) in comparison with control group; 1.49 ± 0.03, P<0.05. Percent inhibition of SOD in TB patients was 11.77 ± 1.98 which was found to be markedly lower than 23.89 ± 0.92 in controls. The levels of lipid peroxidation products; MDA was significantly high in TB patients (4.04±0.25 μmol/l) then control group; 2.03±0.15 μmol/l, P<0.05. Conclusion: The study showed that in TB patients, total antioxidant capacity in terms of TEAC value and SOD in terms of percent inhibition was low while lipid peroxidation products were significantly high. Adequate nutritional supplementation may prove beneficial, representing an efficient approach to fast recovery. |
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| Item Description: | 0974-1143 |