Silver Nanoparticles Synthesized from <i>Abies alba</i> and <i>Pinus sylvestris</i> Bark Extracts: Characterization, Antioxidant, Cytotoxic, and Antibacterial Effects

• Main Authors: Irina Macovei (Author), Simon Vlad Luca (Author), Krystyna Skalicka-Woźniak (Author), Cristina Elena Horhogea (Author), Cristina Mihaela Rimbu (Author), Liviu Sacarescu (Author), Gabriela Vochita (Author), Daniela Gherghel (Author), Bianca Laura Ivanescu (Author), Alina Diana Panainte (Author), Constantin Nechita (Author), Andreia Corciova (Author), Anca Miron (Author)
• Format: Book
• Published: MDPI AG, 2023-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

In recent years, phytofunctionalized AgNPs have attracted great interest due to their remarkable biological activities. In the present study, AgNPs were synthesized using <i>Abies alba</i> and <i>Pinus sylvestris</i> bark extracts. The chemical profile of these bark extracts was analyzed by LC-HRMS/MS. As a first step, the synthesis parameters (pH, AgNO₃ concentration, ratio of bark extract and AgNO₃, temperature, and reaction time) were optimized. The synthesized AgNPs were characterized by ATR-FTIR spectroscopy, DLS, SEM, EDX, and TEM. Their antioxidant, cytotoxic, and antibacterial properties were evaluated by the DPPH, ABTS, MTT, and broth microdilution assays, respectively. <i>Abies alba</i> and <i>Pinus sylvestris</i> bark extract-derived AgNPs were well-dispersed, spherical, small (average particle size of 9.92 and 24.49 nm, respectively), stable (zeta potential values of −10.9 and −10.8 mV, respectively), and cytotoxic to A-375 human malignant melanoma cells (IC₅₀ = 2.40 ± 0.21 and 6.02 ± 0.61 μg/mL, respectively). The phytosynthesized AgNPs also showed antioxidant and antibacterial effects.

Item Description

10.3390/antiox12040797
2076-3921