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Clonal Clusters, Molecular Resistance Mechanisms and Virulence Factors of Gram-Negative Bacteria Isolated from Chronic Wounds in Ghana

Wound infections are common medical problems in sub-Saharan Africa but data on the molecular epidemiology are rare. Within this study we assessed the clonal lineages, resistance genes and virulence factors of Gram-negative bacteria isolated from Ghanaian patients with chronic wounds. From a previous...

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Main Authors: Denise Dekker (Author), Frederik Pankok (Author), Thorsten Thye (Author), Stefan Taudien (Author), Kwabena Oppong (Author), Charity Wiafe Akenten (Author), Maike Lamshöft (Author), Anna Jaeger (Author), Martin Kaase (Author), Simone Scheithauer (Author), Konstantin Tanida (Author), Hagen Frickmann (Author), Jürgen May (Author), Ulrike Loderstädt (Author)
Format: Book
Published: MDPI AG, 2021-03-01T00:00:00Z.
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Summary:Wound infections are common medical problems in sub-Saharan Africa but data on the molecular epidemiology are rare. Within this study we assessed the clonal lineages, resistance genes and virulence factors of Gram-negative bacteria isolated from Ghanaian patients with chronic wounds. From a previous study, 49 <i>Pseudomonas aeruginosa</i>, 21 <i>Klebsiella</i><i>pneumoniae</i> complex members and 12 <i>Escherichia coli</i> were subjected to whole genome sequencing. Sequence analysis indicated high clonal diversity with only nine <i>P. aeruginosa</i> clusters comprising two strains each and one <i>E. coli</i> cluster comprising three strains with high phylogenetic relationship suggesting nosocomial transmission. Acquired beta-lactamase genes were observed in some isolates next to a broad spectrum of additional genetic resistance determinants. Phenotypical expression of extended-spectrum beta-lactamase activity in the Enterobacterales was associated with <i>bla<sub>CTX-M-15</sub></i> genes, which are frequent in Ghana. Frequently recorded virulence genes comprised genes related to invasion and iron-uptake in <i>E. coli</i>, genes related to adherence, iron-uptake, secretion systems and antiphagocytosis in <i>P. aeruginosa</i> and genes related to adherence, biofilm formation, immune evasion, iron-uptake and secretion systems in <i>K. pneumonia</i> complex. In summary, the study provides a piece in the puzzle of the molecular epidemiology of Gram-negative bacteria in chronic wounds in rural Ghana.
Item Description:10.3390/antibiotics10030339
2079-6382

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