Clonal Clusters, Molecular Resistance Mechanisms and Virulence Factors of Gram-Negative Bacteria Isolated from Chronic Wounds in Ghana
Wound infections are common medical problems in sub-Saharan Africa but data on the molecular epidemiology are rare. Within this study we assessed the clonal lineages, resistance genes and virulence factors of Gram-negative bacteria isolated from Ghanaian patients with chronic wounds. From a previous...
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MDPI AG,
2021-03-01T00:00:00Z.
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| 001 | doaj_3fd864dc4c0b4cc0ad2d6b13d1c20d11 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Denise Dekker |e author |
| 700 | 1 | 0 | |a Frederik Pankok |e author |
| 700 | 1 | 0 | |a Thorsten Thye |e author |
| 700 | 1 | 0 | |a Stefan Taudien |e author |
| 700 | 1 | 0 | |a Kwabena Oppong |e author |
| 700 | 1 | 0 | |a Charity Wiafe Akenten |e author |
| 700 | 1 | 0 | |a Maike Lamshöft |e author |
| 700 | 1 | 0 | |a Anna Jaeger |e author |
| 700 | 1 | 0 | |a Martin Kaase |e author |
| 700 | 1 | 0 | |a Simone Scheithauer |e author |
| 700 | 1 | 0 | |a Konstantin Tanida |e author |
| 700 | 1 | 0 | |a Hagen Frickmann |e author |
| 700 | 1 | 0 | |a Jürgen May |e author |
| 700 | 1 | 0 | |a Ulrike Loderstädt |e author |
| 245 | 0 | 0 | |a Clonal Clusters, Molecular Resistance Mechanisms and Virulence Factors of Gram-Negative Bacteria Isolated from Chronic Wounds in Ghana |
| 260 | |b MDPI AG, |c 2021-03-01T00:00:00Z. | ||
| 500 | |a 10.3390/antibiotics10030339 | ||
| 500 | |a 2079-6382 | ||
| 520 | |a Wound infections are common medical problems in sub-Saharan Africa but data on the molecular epidemiology are rare. Within this study we assessed the clonal lineages, resistance genes and virulence factors of Gram-negative bacteria isolated from Ghanaian patients with chronic wounds. From a previous study, 49 <i>Pseudomonas aeruginosa</i>, 21 <i>Klebsiella</i><i>pneumoniae</i> complex members and 12 <i>Escherichia coli</i> were subjected to whole genome sequencing. Sequence analysis indicated high clonal diversity with only nine <i>P. aeruginosa</i> clusters comprising two strains each and one <i>E. coli</i> cluster comprising three strains with high phylogenetic relationship suggesting nosocomial transmission. Acquired beta-lactamase genes were observed in some isolates next to a broad spectrum of additional genetic resistance determinants. Phenotypical expression of extended-spectrum beta-lactamase activity in the Enterobacterales was associated with <i>bla<sub>CTX-M-15</sub></i> genes, which are frequent in Ghana. Frequently recorded virulence genes comprised genes related to invasion and iron-uptake in <i>E. coli</i>, genes related to adherence, iron-uptake, secretion systems and antiphagocytosis in <i>P. aeruginosa</i> and genes related to adherence, biofilm formation, immune evasion, iron-uptake and secretion systems in <i>K. pneumonia</i> complex. In summary, the study provides a piece in the puzzle of the molecular epidemiology of Gram-negative bacteria in chronic wounds in rural Ghana. | ||
| 546 | |a EN | ||
| 690 | |a wounds | ||
| 690 | |a Gram-negative bacteria | ||
| 690 | |a colonization | ||
| 690 | |a infection | ||
| 690 | |a clonal lineages | ||
| 690 | |a resistance genes | ||
| 690 | |a Therapeutics. Pharmacology | ||
| 690 | |a RM1-950 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Antibiotics, Vol 10, Iss 3, p 339 (2021) | |
| 787 | 0 | |n https://www.mdpi.com/2079-6382/10/3/339 | |
| 787 | 0 | |n https://doaj.org/toc/2079-6382 | |
| 856 | 4 | 1 | |u https://doaj.org/article/3fd864dc4c0b4cc0ad2d6b13d1c20d11 |z Connect to this object online. |