Antibacterial Activity of Brown Macroalgae Lipid (Sargassum duplicatum) to The Methicillin Resistant Staphylococcus aureus (MRSA) And Shigella dysentriae Bacteria

• Main Authors: Riong Seulina Panjaitan (Author), Ani Rombe Sarungngu' (Author), Lika Lastri Sitorus (Author)
• Format: Book
• Published: Universitas Sultan Ageng Tirtayasa, 2020-04-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

<em><span lang="IN">Methicillin Resistant Staphylococcus aureus</span></em><span lang="IN"> (MRSA) is the main cause of nosocomial infections in burn patients</span><span lang="IN">due to the occurrence of antibiotic resistance. Dysentery or shigellosis is an infection that causes sores (ulcers) in the large intestine in the middle caused by the bacteria <em>Shigella dysentriae</em>. Some studies show that <em>Sargassum duplicatum</em> has antibacterial abilities. This study aim</span><span lang="EN-US">s</span><span lang="EN-US">to </span><span lang="IN">determine the antibacterial activity of <em>Sargassum duplicatum</em> seaweed lipid extract to the bacteria <em>Methicillin Resistant Staphylococcus aureus</em> (MRSA) and <em>Shigella dysentriae </em>bacteria. The method used in this study was the Folch method (using sochletation with a solvent ratio of chloroform:methanol (2:1)) to extract lipids from <em>Sargassum duplicatum</em>. Then using </span><span lang="EN-US">the </span><span lang="IN">paper disc diffusion method with positive control of tetracycline. From the extraction results obtained</span><span lang="EN-US">, </span><span lang="IN">the lipid levels of <em>Sargassum duplicatum</em>, where the chloroform phase was 0.493% (b / b) and the methanol phase was 2.40% (b / b). The results of the lipid antibacterial activity of <em>Sargassum duplicatum</em> at </span><span lang="EN-US">the </span><span lang="IN">pure concentration in the methanol phase with an average concentration of 7.07 mm on the <em>Methicillin Resistant Staphylococcus aureus</em> (MRSA) Bacteria while in the <em>S</em></span><em><span lang="EN-US">h</span><span lang="IN">igella dysentriae</span></em><span lang="IN"> bacteria obtained an average of 7.05 mm and the chloroform phase obtained an average diameter of 9.16 mm on <em>Methicillin Resistant Staphylococcus aureus</em> (MRSA) and in <em>Shigella dysentriae</em> Bacteria an average of 3.99 mm while tetracycline antibiotics amounted to 11.67 mm on <em>Methicillin Resistant Staphylococcus aureus</em> (MRSA) and 18.98 mm in <em>Shigella dysentriae</em> bacteria.</span>

Item Description

2502-4779
2502-4787
10.30870/educhemia.v5i1.6432