An Organic Fraction of <i>Oenothera rosea</i> L'Her Ex. Aiton Prevents Neuroinflammation in a Rat Ischemic Model
Background: <i>Oenothera rosea</i> L'Her Ex. Aiton, presenting antioxidant and anti-inflammatory activities, is traditionally used to treat bruises and headaches and as a healing agent. This study aimed to investigate whether its organic fraction (EAOr) has neuroprotective propertie...
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| Main Authors: | , , , , , , |
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| Format: | Book |
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MDPI AG,
2024-09-01T00:00:00Z.
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| Summary: | Background: <i>Oenothera rosea</i> L'Her Ex. Aiton, presenting antioxidant and anti-inflammatory activities, is traditionally used to treat bruises and headaches and as a healing agent. This study aimed to investigate whether its organic fraction (EAOr) has neuroprotective properties against neuroinflammation in the context of ischemia/reperfusion. Methods: The chemical composition of EAOr was determined using HPLC techniques, and its neuroprotective activities were evaluated in a common carotid-artery ligation model for the induction of ischemia/reperfusion (I/R). The animals were supplemented with EAOR for 15 days. On the last day, the animals were rested for one hour, following which the common carotid-artery ligation procedure was performed to induce I/R. The neurological deficit was evaluated at 24 h after I/R using Bederson's scale, and the relative expression of inflammatory genes and structure of hippocampal neurons were analyzed at 48 h. Results: The chemical analysis revealed five major compounds in EAOr: gallic acid, rutin, ellagic acid, and glucoside and rhamnoside quercetin. EAOr prevented neurological deficit 24 h after I/R; led to the early activation of the <i>AIF</i> and <i>GFAP</i> genes; reduced <i>Nfkb1</i>, <i>IL-1beta</i>, <i>Il-6</i> and <i>Casp3</i> gene expression; and protected hippocampal neurons. Conclusions: Our findings demonstrate that EAOr contains polyphenol-type compounds, which could exert a therapeutic effect through the inhibition of neuroinflammation and neuronal death genes, thus maintaining hippocampal neurons. |
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| Item Description: | 10.3390/ph17091184 1424-8247 |