Efficiency of cleaning and disinfection of surfaces: correlation between assessment methods

• Main Authors: Oleci Pereira Frota (Author), Adriano Menis Ferreira (Author), Odanir Garcia Guerra (Author), Marcelo Alessandro Rigotti (Author), Denise de Andrade (Author), Najla Moreira Amaral Borges (Author), Margarete Teresa Gottardo de Almeida (Author)
• Format: Book
• Published: Associação Brasileira de Enfermagem.
• Subjects: article
• Online Access: Connect to this object online.

Summary

ABSTRACT Objective: to assess the correlation among the ATP-bioluminescence assay, visual inspection and microbiological culture in monitoring the efficiency of cleaning and disinfection (C&D) of high-touch clinical surfaces (HTCS) in a walk-in emergency care unit. Method: a prospective and comparative study was carried out from March to June 2015, in which five HTCS were sampled before and after C&D by means of the three methods. The HTCS were considered dirty when dust, waste, humidity and stains were detected in visual inspection; when ≥2.5 colony forming units per cm2 were found in culture; when ≥5 relative light units per cm2 were found at the ATP-bioluminescence assay. Results: 720 analyses were performed, 240 per method. The overall rates of clean surfaces per visual inspection, culture and ATP-bioluminescence assay were 8.3%, 20.8% and 44.2% before C&D, and 92.5%, 50% and 84.2% after C&D, respectively (p<0.001). There were only occasional statistically significant relationships between methods. Conclusion: the methods did not present a good correlation, neither quantitative nor qualitatively.

Item Description

1984-0446
10.1590/0034-7167-2016-0608