Evaluation of viral load in saliva from patients with chronic hepatitis C infection
Summary: Hepatitis C virus can be detected in blood and other bodily fluids, such as saliva. The aim of this study was to detect and quantify the HCV-RNA in saliva and plasma from patients with chronic hepatitis C infections, as well as check the level of viral load in sex groups (age, ethnicity and...
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| Main Authors: | , , , , |
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| Format: | Book |
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Elsevier,
2015-09-01T00:00:00Z.
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| Summary: | Summary: Hepatitis C virus can be detected in blood and other bodily fluids, such as saliva. The aim of this study was to detect and quantify the HCV-RNA in saliva and plasma from patients with chronic hepatitis C infections, as well as check the level of viral load in sex groups (age, ethnicity and virus subtypes). Whole saliva and blood from 70 patients with chronic hepatitis C infections attended at the department of gastroenterology from University Hospital. The HCV-RNA load was performed by qRT-PCR using Sybr Green I master mix. HCV-RNA was detected in 80% (56/70) of patients in saliva and 92.85% (65/70) in plasma. The median of the viral load in the plasma was of 4.87 log10, and in saliva, it was 3.32 log10, (p = 0.0005). Female patients and black patients exhibited a negative correlation between the HCV-RNA load in saliva vs. the HCV-RNA load in plasma (r = −0.3172, CI95% −0.6240 to −0.03736, p = 0.0491) and (r = −0.3141; IC95% −0.6069 to −0.05926; p = 0.0209), respectively. HCV-RNA was detected and quantified in saliva samples, and according to the quantification levels, saliva may be a possible transmission source of HCV, particularly in women and people of black ethnicity who develop chronic HCV infections. Keywords: Chronic hepatitis C infection, qPCR, Viral subtype, Ethnicity |
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| Item Description: | 1876-0341 10.1016/j.jiph.2015.04.025 |