Ethanolic Extract of <i>Ocimum sanctum</i> Linn. Inhibits Cell Migration of Human Lung Adenocarcinoma Cells (A549) by Downregulation of Integrin αvβ3, α5β1, and VEGF

• Główni autorzy: Ulayatul Kustiati (Autor), Suleyman Ergün (Autor), Srikanth Karnati (Autor), Dwi Aris Agung Nugrahaningsih (Autor), Dwi Liliek Kusindarta (Autor), Hevi Wihadmadyatami (Autor)
• Format: Książka
• Wydane: MDPI AG, 2022-10-01T00:00:00Z.
• Hasła przedmiotowe: article
• Dostęp online: Connect to this object online.

Streszczenie

Adenocarcinoma lung cancer is a type of non-small cell lung carcinoma (NSCLC), which accounts for 85% of lung cancer incidence globally. The therapies that are being applied, both conventional therapies and antibody-based treatments, are still found to have side effects. Several previous studies have demonstrated the ability of the ethanolic extract of <i>Ocimum sanctum</i> Linn. (EEOS) as an ethnomedicine with anti-tumor properties. The aim of this study was to determine the effect of <i>Ocimum sanctum</i> Linn. ethanolic extract in inhibiting the proliferation, angiogenesis, and migration of A549 cells (NSCLC). The adhesion as well as the migration assay was performed. Furthermore, enzyme-linked immunosorbent assay (ELISA) was used to measure the expression of αvβ3 integrins, α5β1 integrins, and VEGF. The cells were divided into the following treatment groups: control (non-treated/NT), positive control (AP3/inhibitor β3 80 µg/mL), cisplatin (9 µg/mL), and EEOS at concentrations of 50, 70, 100, and 200 µg/mL. The results showed that EEOS inhibits the adhesion ability and migration of A549 cells, with an optimal concentration of 200 µg/mL. ELISA testing showed that the group of A549 cells given EEOS 200 µg/mL presented a decrease in the optimal expression of integrin α5β1, integrin αvβ3, and VEGF.

Deskrypcja

10.3390/scipharm90040069
2218-0532
0036-8709