Simultaneous Real-Time Detection of Initiator- and Effector-Caspase Activation by Double Fluorescence Resonance Energy Transfer Analysis

• Main Authors: Hiroshi Kawai (Author), Takuo Suzuki (Author), Tetsu Kobayashi (Author), Haruna Sakurai (Author), Hisayuki Ohata (Author), Kazuo Honda (Author), Kazutaka Momose (Author), Iyuki Namekata (Author), Hikaru Tanaka (Author), Koki Shigenobu (Author), Ryu Nakamura (Author), Takao Hayakawa (Author), Toru Kawanishi (Author)
• Format: Book
• Published: Elsevier, 2005-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Fluorescence resonance energy transfer (FRET) with green fluorescent protein (GFP) variants has become widely used for biochemical research. In order to expand the choice of fluorescent range in FRET analysis, we designed various color versions of the FRET-based probes for caspase activity, in which...