Analysis of Semen Parameters in α1,3-Galactosyltransferase-/- Boars
It is very difficult to get the information about semen quality analysis in transgenic pigs because of limited numbers and research facilities. Therefore, in the present study, we analyzed the semen quality of transgenic boars generated for xenotransplantation research. Briefly, the semen samples we...
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| Main Authors: | , , , , , , , , , , |
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The Korean Society of Animal Reproduction and Biotechnology,
2017-06-01T00:00:00Z.
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| 001 | doaj_4450f5b8452b47c89dee9d03f4b47d9a | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a In-Sul Hwang |e author |
| 700 | 1 | 0 | |a Seung-Chan Lee |e author |
| 700 | 1 | 0 | |a Sung Woo Kim |e author |
| 700 | 1 | 0 | |a Dae-Jin Kwon |e author |
| 700 | 1 | 0 | |a Mi-Ryung Park |e author |
| 700 | 1 | 0 | |a Hyeon Yang |e author |
| 700 | 1 | 0 | |a Keon Bong Oh |e author |
| 700 | 1 | 0 | |a Sun-A Ock |e author |
| 700 | 1 | 0 | |a Jae-Seok Woo |e author |
| 700 | 1 | 0 | |a Gi-Sun Im |e author |
| 700 | 1 | 0 | |a Seongsoo Hwang |e author |
| 245 | 0 | 0 | |a Analysis of Semen Parameters in α1,3-Galactosyltransferase-/- Boars |
| 260 | |b The Korean Society of Animal Reproduction and Biotechnology, |c 2017-06-01T00:00:00Z. | ||
| 500 | |a 10.12750/JET.2017.32.2.53 | ||
| 500 | |a 2671-4639 | ||
| 500 | |a 2671-4663 | ||
| 520 | |a It is very difficult to get the information about semen quality analysis in transgenic pigs because of limited numbers and research facilities. Therefore, in the present study, we analyzed the semen quality of transgenic boars generated for xenotransplantation research. Briefly, the semen samples were collected from 5 homozygous α1,3-Galactosyltransferase knock-out (GalT-/-) transgenic boars and immediately transported to the laboratory. These semen samples were decupled with DPBS and conducted to analyze semen parameters by a computer-assisted semen analysis (CASA) system. The boar semen were examined all 12 parameters such as total motility (TM), curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP), and hyperactivated (HYP), etc. In results, among the 5 GalT-/- boars, three boars (#134, 144, and 170) showed normal range of semen parameters, but #199 and 171 boars showed abnormal ranges of semen parameters according to standard ranges of semen parameters. Unfortunately, #171 boar showed azoospermia symptom with rare sperm counts in the original semen. Conclusively, assessment of semen parameters by CASA system is useful to pre-screening of reproductively healthy boar prior to natural mating and artificial insemination for multiplication and breeding. | ||
| 546 | |a EN | ||
| 546 | |a KO | ||
| 690 | |a semen parameters | ||
| 690 | |a casa | ||
| 690 | |a transgenic boars | ||
| 690 | |a xenotransplantation | ||
| 690 | |a Biotechnology | ||
| 690 | |a TP248.13-248.65 | ||
| 690 | |a Medicine (General) | ||
| 690 | |a R5-920 | ||
| 690 | |a Internal medicine | ||
| 690 | |a RC31-1245 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Journal of Animal Reproduction and Biotechnology, Vol 32, Iss 2, Pp 53-58 (2017) | |
| 787 | 0 | |n http://www.e-jarb.org/journal/view.html?uid=15&vmd=Full | |
| 787 | 0 | |n https://doaj.org/toc/2671-4639 | |
| 787 | 0 | |n https://doaj.org/toc/2671-4663 | |
| 856 | 4 | 1 | |u https://doaj.org/article/4450f5b8452b47c89dee9d03f4b47d9a |z Connect to this object online. |