Release test of alliin/alliinase double-layer tablet by HPLCâAllicin determination

• Main Authors: Yan Liang (Author), Jing-Jing Zhang (Author), Qi-Bing Zhang (Author), Zhong-Xia Wang (Author), Zong-Ning Yin (Author), Xin-Xia Li (Author), Jian Chen (Author), Li-Ming Ye (Author)
• Format: Book
• Published: Elsevier, 2013-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

A simple, precise and accurate method was developed and validated for the determination of allicin release from alliin/alliinase double-layer tablets. According to Appendix XC II of Chinese Pharmacopoeia 2010 edition Volume II, a small glass-method was adopted at the rotational speed of 100r/min using 100mL phosphate buffer (pH 6.8) as release medium. The release amount was determined by HPLC with a C18 column (250mmÃ4.6mm, 5μm) using the mobile phase consisting of methanol â0.4% carboxylic acid (65:35) at a flow rate of 1mL/min and UV detection at 242nm. The current method demonstrates good linearity over the range 4.052â405.2μg/mL (r2=0.9999) with an average recovery of 105.5%(RSD=1.25%). The accumulative release of alliin/alliinase double-layer tablets had good homogeneity for within- and between-batches. The method established is simple, accurate and repeatable for the determination of allicin release from alliin/alliinase double-layer tablets. Keywords: Alliin/alliinase, Double-layer tablet, Allicin, Release test, HPLC

Item Description

2095-1779
10.1016/j.jpha.2012.08.001