<i>Streptomyces rugosispiralis</i> sp. nov., a Novel Actinobacterium Isolated from Peat Swamp Forest Soil That Produces Ansamycin Derivatives and Nocardamines
Actinomycetes, especially the genus <i>Streptomyces</i>, are one of the most promising sources of bioactive natural products. In this study, a novel <i>Streptomyces</i> strain, RCU-064<sup>T</sup>, was isolated from a soil sample collected from a peat swamp forest...
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| Main Authors: | , , , , , , , |
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| Format: | Book |
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MDPI AG,
2023-09-01T00:00:00Z.
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| Summary: | Actinomycetes, especially the genus <i>Streptomyces</i>, are one of the most promising sources of bioactive natural products. In this study, a novel <i>Streptomyces</i> strain, RCU-064<sup>T</sup>, was isolated from a soil sample collected from a peat swamp forest in Thailand. Strain RCU-064<sup>T</sup> showed the highest 16S rRNA gene sequence similarity (99.06%) with <i>Streptomyces malaysiensis</i> NBRC 16446<sup>T</sup>. Based on a polyphasic approach, strain RCU-064<sup>T</sup> represents a novel species of the genus <i>Streptomyces</i>, for which the name <i>Streptomyces rugosispiralis</i> sp. nov. is proposed. The chemical isolation of the crude ethyl acetate extracts of the strain led to the isolation of six compounds: (<b>1</b>) geldanamycin, (<b>2</b>) 17-<i>O</i>-demethylgeldanamycin, (<b>3</b>) reblastatin, (<b>4</b>) 17-demethoxyreblastatin, (<b>5</b>) nocardamine, and (<b>6</b>) dehydroxynocardamine. These compounds were evaluated for their biological activities. All compounds showed no antimicrobial activity against tested microorganisms used in this study. Compounds (<b>1</b>)-(<b>4</b>) displayed cytotoxic activity against the NCI-H187 cell line, with IC<sub>50</sub> values ranging from 0.045-4.250 µg/mL. Cytotoxicity against the MCF-7 cell line was found in compounds (<b>1</b>) and (<b>3</b>) with IC<sub>50</sub> values of 3.51 and 1.27 µg/mL, respectively. Compounds (<b>5</b>) and (<b>6</b>) exhibited cytotoxicity only against Vero cells (IC<sub>50</sub> of 16.57 µg/mL) and NCI-H187 cells (IC<sub>50</sub> of 13.96 µg/mL), respectively. These results indicate that peat swamp forest soil remains a promising reservoir of novel actinomycetes capable of producing bioactive natural products. |
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| Item Description: | 10.3390/antibiotics12091467 2079-6382 |