Increased production of hydrogen peroxide by peripheral blood monocytes associated with smoking exposure intensity in smokers
<p>Abstract</p> <p>Background</p> <p>Smoking is known to be associated with oxidative stress; however, it has not been elucidated whether the oxidative response is influenced by the intensity of smoking exposure.</p> <p>Objectives</p> <p>Evaluate...
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| Main Authors: | , , , , , |
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| Format: | Book |
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BMC,
2012-11-01T00:00:00Z.
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| Summary: | <p>Abstract</p> <p>Background</p> <p>Smoking is known to be associated with oxidative stress; however, it has not been elucidated whether the oxidative response is influenced by the intensity of smoking exposure.</p> <p>Objectives</p> <p>Evaluate the effect of smoking exposure on the secretion of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) by the peripheral blood monocytes of smokers.</p> <p>Methods</p> <p>A total of 25 smokers (50.3±8.8 years, 48% male) underwent the following evaluations: spirometry, pulse oximetry, body composition and total peripheral blood count. Peripheral blood monocyte (PBM) cultures were isolated and maintained, and IL-6 and TNF-α were measured in the plasma and in the supernatants of spontaneous and stimulated cultures. H<sub>2</sub>O<sub>2</sub> was evaluated in the supernatants of the PBM cultures, and a subset of the PBM culture supernatants was stimulated with phorbol myristate acetate (PMA). We also evaluated 38 healthy controls (49.1±8.2 years, 42% male).</p> <p>Results</p> <p>The spontaneous and stimulated monocytes' secretion of H<sub>2</sub>O<sub>2</sub> were statistically higher in the smokers than in the healthy controls (p<0.001). The H<sub>2</sub>O<sub>2</sub> secretions were statistically significant higher after stimulation with PMA in both groups (p<0.001). In the multiple regression analysis, we identified a positive, statistically significant association between pack-years of smoking and the spontaneous secretion of H<sub>2</sub>O<sub>2</sub> by PBM culture, adjusted for potential confounding variables. The association between PBM culture secretion of H<sub>2</sub>O<sub>2</sub> and the production of TNF-α and IL-6 was not significant.</p> <p>Conclusion</p> <p>We identified a positive association between higher production of H<sub>2</sub>O<sub>2</sub> in smokers and higher smoking exposure during life. The influence of pack-years smoking may be a key modifiable factor in oxidative stress associated to smoking.</p> |
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| Item Description: | 10.1186/1476-9255-9-45 1476-9255 |