Lipid-based Transfection Reagents Exhibit Cryo-induced Increase in Transfection Efficiency

• Main Authors: Helena Sork (Author), Joel Z Nordin (Author), Janne J Turunen (Author), Oscar PB Wiklander (Author), Burcu Bestas (Author), Eman M Zaghloul (Author), Helerin Margus (Author), Kärt Padari (Author), Adil D Duru (Author), Giulia Corso (Author), Jeremy Bost (Author), Pieter Vader (Author), Margus Pooga (Author), CI Edvard Smith (Author), Matthew JA Wood (Author), Raymond M Schiffelers (Author), Mattias Hällbrink (Author), Samir EL Andaloussi (Author)
• Format: Book
• Published: Elsevier, 2016-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The advantages of lipid-based transfection reagents have permitted their widespread use in molecular biology and gene therapy. This study outlines the effect of cryo-manipulation of a cationic lipid-based formulation, Lipofectamine 2000, which, after being frozen and thawed, showed orders of magnitu...