Lipid-based Transfection Reagents Exhibit Cryo-induced Increase in Transfection Efficiency

• Váldodahkkit: Helena Sork (Dahkki), Joel Z Nordin (Dahkki), Janne J Turunen (Dahkki), Oscar PB Wiklander (Dahkki), Burcu Bestas (Dahkki), Eman M Zaghloul (Dahkki), Helerin Margus (Dahkki), Kärt Padari (Dahkki), Adil D Duru (Dahkki), Giulia Corso (Dahkki), Jeremy Bost (Dahkki), Pieter Vader (Dahkki), Margus Pooga (Dahkki), CI Edvard Smith (Dahkki), Matthew JA Wood (Dahkki), Raymond M Schiffelers (Dahkki), Mattias Hällbrink (Dahkki), Samir EL Andaloussi (Dahkki)
• Materiálatiipa: Girji
• Almmustuhtton: Elsevier, 2016-01-01T00:00:00Z.
• Fáttát: article
• Liŋkkat: Connect to this object online.

The advantages of lipid-based transfection reagents have permitted their widespread use in molecular biology and gene therapy. This study outlines the effect of cryo-manipulation of a cationic lipid-based formulation, Lipofectamine 2000, which, after being frozen and thawed, showed orders of magnitu...