<it>In vitro </it>cytotoxicity of <it>Strobilanthes crispus </it>ethanol extract on hormone dependent human breast adenocarcinoma MCF-7 cell

• Main Authors: Chong Hueh (Author), Rahmat Asmah (Author), Yeap Swee (Author), Md Akim Abdah (Author), Alitheen Noorjahan (Author), Othman Fauziah (Author), Gwendoline-Ee Cheng (Author)
• Format: Book
• Published: BMC, 2012-04-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

<p>Abstract</p> <p>Background</p> <p><it>Strobilanthes crispus </it>has been traditionally used as antidiabetic, anticancer, diuretic, antilytic and laxative agent. However, cytotoxicity and antiproliferative effect of <it>S. crispus </it>is still unclear.</p> <p>Results</p> <p><it>Strobilanthes cripus </it>was able to reduce cell viability and proliferation in MTT and BrdU assays. Both cell cycle progression and Tunel assay suggested that IC₅₀of <it>S. crispus </it>ethanol extract induced sub-G1 cell cycle phase, and DNA fragmentation. On the other hand, translocation of mitochondria cytochrome c release, induction of caspase 3/7 and p53 while suppress XIAP on treated MCF-7 cell were also observed in this study.</p> <p>Conclusion</p> <p>Our findings suggest that <it>S. crispus </it>ethanol extract induced apoptosis and DNA fragmentation on hormone dependent breast cancer cell line MCF-7 via mitochondria dependent p53 apoptosis pathway.</p>

Item Description

10.1186/1472-6882-12-35
1472-6882