<it>Kalanchoe tubiflora</it> extract inhibits cell proliferation by affecting the mitotic apparatus
<p>Abstract</p> <p>Background</p> <p><it>Kalanchoe tubiflora</it> (KT) is a succulent plant native to Madagascar, and is commonly used as a medicinal agent in Southern Brazil. The underlying mechanisms of tumor suppression are largely unexplored.</p> &...
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| Main Authors: | , , , , , , |
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| Format: | Book |
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BMC,
2012-09-01T00:00:00Z.
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| Summary: | <p>Abstract</p> <p>Background</p> <p><it>Kalanchoe tubiflora</it> (KT) is a succulent plant native to Madagascar, and is commonly used as a medicinal agent in Southern Brazil. The underlying mechanisms of tumor suppression are largely unexplored.</p> <p>Methods</p> <p>Cell viability and wound-healing were analyzed by MTT assay and scratch assay respectively. Cell cycle profiles were analyzed by FACS. Mitotic defects were analyzed by indirect immunofluoresence images.</p> <p>Results</p> <p>An n-Butanol-soluble fraction of KT (KT-NB) was able to inhibit cell proliferation. After a 48 h treatment with 6.75 μg/ml of KT, the cell viability was less than 50% of controls, and was further reduced to less than 10% at higher concentrations. KT-NB also induced an accumulation of cells in the G2/M phase of the cell cycle as well as an increased level of cells in the subG1 phase. Instead of disrupting the microtubule network of interphase cells, KT-NB reduced cell viability by inducing multipolar spindles and defects in chromosome alignment. KT-NB inhibits cell proliferation and reduces cell viability by two mechanisms that are exclusively involved with cell division: first by inducing multipolarity; second by disrupting chromosome alignment during metaphase.</p> <p>Conclusion</p> <p>KT-NB reduced cell viability by exclusively affecting formation of the proper structure of the mitotic apparatus. This is the main idea of the new generation of anti-mitotic agents. All together, KT-NB has sufficient potential to warrant further investigation as a potential new anticancer agent candidate.</p> |
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| Item Description: | 10.1186/1472-6882-12-149 1472-6882 |