Potential of [<sup>11</sup>C](<i>R</i>)-PK11195 PET Imaging for Evaluating Tumor Inflammation: A Murine Mammary Tumor Model
Background: Breast tumor inflammation is an immunological process that occurs mainly by mediation of Tumor-Associated Macrophages (TAM). Aiming for a specific measurement of tumor inflammation, the current study evaluated the potential of Positron Emission Tomography (PET) imaging with [<sup>1...
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Main Authors: | , , , , , , , , |
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Format: | Book |
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MDPI AG,
2022-12-01T00:00:00Z.
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Summary: | Background: Breast tumor inflammation is an immunological process that occurs mainly by mediation of Tumor-Associated Macrophages (TAM). Aiming for a specific measurement of tumor inflammation, the current study evaluated the potential of Positron Emission Tomography (PET) imaging with [<sup>11</sup>C](<i>R</i>)-PK11195 to evaluate tumor inflammation in a mammary tumor animal model. Methods: Female Balb/C mice were inoculated with 4T1 cells. The PET imaging with [<sup>11</sup>C](<i>R</i>)-PK11195 and [<sup>18</sup>F]FDG was acquired 3 days, 1 week, and 2 weeks after cell inoculation. Results: The [<sup>11</sup>C](<i>R</i>)-PK11195 tumor uptake increased from 3 days to 1 week, and decreased at 2 weeks after cell inoculation, as opposed to the [<sup>18</sup>F]FDG uptake, which showed a slight decrease in uptake at 1 week and increased uptake at 2 weeks. In the control group, no significant differences occurred in tracer uptake over time. Tumor uptake of both radiopharmaceuticals is more expressed in tumor edge regions, with greater intensity at 2 weeks, as demonstrated by [<sup>11</sup>C](<i>R</i>)-PK11195 autoradiography and immunofluorescence with TSPO antibodies and CD86 pro-inflammatory phenotype. Conclusion: The [<sup>11</sup>C](<i>R</i>)-PK11195 was able to identify heterogeneous tumor inflammation in a murine model of breast cancer and the uptake varied according to tumor size. Together with the glycolytic marker [<sup>18</sup>F]FDG, molecular imaging with [<sup>11</sup>C](<i>R</i>)-PK11195 may provide a better characterization of inflammatory responses in cancer. |
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Item Description: | 10.3390/pharmaceutics14122715 1999-4923 |