Procedure for the Screening of Eggs and Egg Products to Detect Oxolonic Acid, Ciprofloxacin, Enrofloxacin, and Sarafloxacin Using Micellar Liquid Chromatography

• Main Authors: Juan Peris-Vicente (Author), Daniel García-Ferrer (Author), Pooja Mishra (Author), Jaume Albiol-Chiva (Author), Abhilasha Durgbanshi (Author), Samuel Carda-Broch (Author), Devasish Bose (Author), Josep Esteve-Romero (Author)
• Format: Book
• Published: MDPI AG, 2019-11-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 2079-6382; 10.3390/antibiotics8040226
• Keywords: food safety; fluorescence; laying hen; optimization; solid-to-liquid extraction; validation; Therapeutics. Pharmacology; RM1-950
• Source: Antibiotics, Vol 8, Iss 4, p 226 (2019)

A method based on micellar liquid chromatography was developed to determine oxolinic acid, ciprofloxacin, enrofloxacin, and sarafloxacin in eggs and egg products. The antimicrobial drugs were obtained in a micellar solution which was directly injected. The analytes were resolved using a C18 column and a mobile phase of 0.05 M sodium dodecyl sulfate-7.5% 1-propanol-0.5% triethylamine, buffered at pH 3 with phosphate salt, running under the isocratic mode. The signal was monitored by fluorescence. Validation was successfully performed according to the EU Commission Decision 2002/657/EC in terms of specificity, calibration range (LOQ to 1 mg/kg), linearity (<i>R</i>² > 0.9991), limit of detection and decision limit (0.01-0.05 mg/kg), limit of quantification (0.025-0.150 mg/kg), detection capability (<0.4 times decision limit), trueness (−14.2% to +9.8%), precision (<14.0%), robustness, and stability. The procedure was environmentally friendly, safe, easy-to-conduct, inexpensive, and had a high sample throughput, thus it is useful for routine analysis as a screening method in a laboratory for food residue control.