In Vitro Antidiabetic, Antioxidant, and Prebiotic Activities of the Chemical Compounds Isolated from <i>Guizotia abyssinica</i>
India and Ethiopia employ <i>Guizotia abyssinica</i> (niger plant) as a source of edible vegetable oil. Previous studies have documented the niger plant's antioxidant properties and dietary benefits. Here, <i>G. abyssinica</i> extract was obtained and ten known bioactive...
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| Main Authors: | , , , , , , , |
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| Format: | Book |
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MDPI AG,
2022-12-01T00:00:00Z.
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| Summary: | India and Ethiopia employ <i>Guizotia abyssinica</i> (niger plant) as a source of edible vegetable oil. Previous studies have documented the niger plant's antioxidant properties and dietary benefits. Here, <i>G. abyssinica</i> extract was obtained and ten known bioactive components (<b>1</b>-<b>10</b>) were isolated. The antioxidant, antidiabetic, and prebiotic properties of whole extract and isolated components of niger and the plant's ability to cooperate symbiotically with probiotic strains were examined. Compound <b>10</b>, myricetin-3-<i>O</i>-L-rhamnoside, had the highest antioxidant capacity measured in the 2,2-diphenylpicrylhydrazyl (DPPH, 4629.76 ± 6.02 µmol Trolox equivalent/g compound) and ferric-reducing antioxidant power (FRAP, 2667.62 ± 7.5 mol Trolox equivalent/g compound) assays. The lowest <i>α</i>-amylase and glycogen phosphorylase activities and glucose diffusion were obtained with whole <i>G. abyssinica</i> extracts, whereas compounds <b>8</b>-<b>10</b> had moderate inhibitory effects. <i>G. abyssinica</i> extract also induced the highest glucose absorption by yeast cells in the presence of 5 mM of glucose. Moreover, <i>Lactobacillus plantarum</i> and <i>L. rhamnosus</i> incubated with <i>β</i>-sitosterol 3-<i>O</i>-D-glucoside (compound <b>7</b>) showed the highest prebiotic activity score. The levels of L-(+)-lactic acid isomer in the probiotic strains were the highest in presence of the whole extract and decreased progressively in the presence of flavonoid glycosides (compounds <b>8</b>-<b>10</b>) and <i>β</i>-sitosterol 3-<i>O</i>-D-glucoside. The enzymatic profile of the probiotic strains was unaffected by the niger extract and compounds <b>7</b>-<b>10</b>. The findings revealed that the biological activities of <i>G. abyssinica</i> extract are mediated by the compounds <b>1</b>-<b>10</b>, and it may be considered as a promising plant for the treatment of diabetes mellitus<b>.</b> |
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| Item Description: | 10.3390/antiox11122482 2076-3921 |