Concordance between Antimicrobial Resistance Phenotype and Genotype of <i>Staphylococcus pseudintermedius</i> from Healthy Dogs
<i>Staphylococcus pseudintermedius</i>, a common commensal canine bacterium, is the main cause of skin infections in dogs and is a potential zoonotic pathogen. The emergence of methicillin-resistant <i>S. pseudintermedius</i> (MRSP) has compromised the treatment of infections...
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| Main Authors: | , , , , , , , |
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| Format: | Book |
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MDPI AG,
2022-11-01T00:00:00Z.
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| Online Access: | Connect to this object online. |
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| Summary: | <i>Staphylococcus pseudintermedius</i>, a common commensal canine bacterium, is the main cause of skin infections in dogs and is a potential zoonotic pathogen. The emergence of methicillin-resistant <i>S. pseudintermedius</i> (MRSP) has compromised the treatment of infections caused by these bacteria. In this study, we compared the phenotypic results obtained by minimum inhibitory concentration (MICs) for 67 <i>S. pseudintermedius</i> isolates from the skin of nine healthy dogs versus the genotypic data obtained with Nanopore sequencing. A total of 17 antibiotic resistance genes (ARGs) were detected among the isolates. A good correlation between phenotype and genotype was observed for some antimicrobial classes, such as ciprofloxacin (fluoroquinolone), macrolides, or tetracycline. However, for oxacillin (beta-lactam) or aminoglycosides the correlation was low. Two antibiotic resistance genes were located on plasmids integrated in the chromosome, and a third one was in a circular plasmid. To our knowledge, this is the first study assessing the correlation between phenotype and genotype regarding antimicrobial resistance of <i>S. pseudintermedius</i> from healthy dogs using Nanopore sequencing technology. |
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| Item Description: | 10.3390/antibiotics11111625 2079-6382 |