Influence of substratum position and acquired pellicle on <italic>Candida albicans</italic> biofilm
<p>The purpose of this study was to evaluate the influence of the substratum position and the saliva acquired pellicle (AP) on <italic>Candida albicans</italic> biofilm development. Poly(methylmethacrylate) (PMMA) disks were fabricated and randomly allocated to experimental groups:...
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| Main Authors: | , , , , |
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| Format: | Book |
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Sociedade Brasileira de Pesquisa Odontológica,
2013-11-01T00:00:00Z.
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| Summary: | <p>The purpose of this study was to evaluate the influence of the substratum position and the saliva acquired pellicle (AP) on <italic>Candida albicans</italic> biofilm development. Poly(methylmethacrylate) (PMMA) disks were fabricated and randomly allocated to experimental groups: HNP (disks placed in a horizontal position and uncoated by pellicle), VNP (disks placed in a vertical position and uncoated by pellicle), HCP (disks placed in a horizontal position and coated by pellicle), and VCP (disks placed in a vertical position and coated by pellicle). Disks were placed in a 24-well plate and a suspension of 10<sup>7 </sup>cells/mL of <italic>Candida albicans</italic> was added to each well for biofilm development. The plates were aerobically incubated at 35°C. The biofilms were evaluated at 1.5 (adhesion time point), 24, 48, 72, and 96 hours. The number of viable cells was quantified in terms of the colony-forming units per milliliter (CFU/mL). Metabolic activity was measured by the XTT assay. The biofilm structure was analyzed by scanning electron microscopy. The data were analyzed by three-way ANOVA followed by Tukey's test, with significance set at 5%. The vertical groups showed less biofilm formation and lower metabolic activity than the horizontal groups (<italic>p</italic>< 0.05). Significant differences in cell viability and metabolic activity were observed between the adhesion and other time points (<italic>p</italic>< 0.05), but these variables were not affected by the presence of the pellicle (<italic>p</italic> > 0.05). It can be concluded that the substratum position influenced biofilm development.</p> |
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| Item Description: | 1806-8324 |