Optimization of sand fly embryo microinjection for gene editing by CRISPR/Cas9.

• Main Authors: Ines Martin-Martin (Author), Azadeh Aryan (Author), Claudio Meneses (Author), Zach N Adelman (Author), Eric Calvo (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2018-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

BACKGROUND:Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 technology has rapidly emerged as a very effective tool for gene editing. Although great advances on gene editing in the medical entomology field have arisen, no attempts of gene editing have been reported in sand fli...