BIChromET: A Chromogenic Culture Medium for Detection of Piperacillin/Tazobactam and Cefepime Resistance in <i>Pseudomonas aeruginosa</i>

• Main Authors: José Manuel Ortiz de la Rosa (Author), Ángel Rodríguez-Villodres (Author), Guillermo Martín-Gutiérrez (Author), Carmen Cintora Mairal (Author), José Luis García Escobar (Author), Lydia Gálvez-Benítez (Author), José Miguel Cisneros (Author), José Antonio Lepe (Author)
• Format: Book
• Published: MDPI AG, 2023-10-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Objectives: The BIChromET selective medium for detecting piperacillin-tazobactam (TZP) and cefepime (FEP) resistant <i>Pseudomonas aeruginosa</i> was developed. Methods: The performance of this medium was first evaluated using a collection of 100 <i>P. aeruginosa</i> clinical strains (70 TZP-susceptible, 30 TZP-resistant, 58 FEP-susceptible, and 42 FEP-resistant). Then, we performed clinical validation by testing 173 respiratory clinical samples. Results: The BIChromET medium showed excellent sensitivity (TZP (avg. 96.7%); FEP (avg. 92.7%)) and specificity (TZP (avg. 98.9%); FEP (avg. 98%)) in distinguishing the detection limit ranging from 10⁴ to 10⁸ CFU/mL. Then, testing the bronchoalveolar lavage (BAL) and tracheobronchial aspirate (TBA) clinical specimens (N = 173) revealed the excellent performance of the medium with <i>P. aeruginosa</i>, showing 100% and 92.6% of categorical agreements with the results obtained via the broth microdilution methods (BMD) for TZP and FEP, respectively. Conclusion: This medium allows for easy and accurate detection of TZP/FEP-resistant isolates regardless of their resistance mechanisms.

Item Description

10.3390/antibiotics12111573
2079-6382