A Microneedle-Microplate Platform to Detect Biomarkers in the Skin
This research aimed to integrate an established signal quantification technique with immunocapture microneedle arrays (MNAs) to improve biomarker-based disease diagnostics in the skin. In this miniaturized sandwich enzyme-linked immunosorbent assay (ELISA) platform, the capture antibody was immobili...
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| Format: | Book |
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University of Huddersfield Press,
2022-11-01T00:00:00Z.
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| LEADER | 00000 am a22000003u 4500 | ||
|---|---|---|---|
| 001 | doaj_54d0a1a14979443d82c97a6870d82da0 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Rachael Victoria Dixon |e author |
| 700 | 1 | 0 | |a Wing Man Lau |e author |
| 700 | 1 | 0 | |a Keng Wooi Ng |e author |
| 245 | 0 | 0 | |a A Microneedle-Microplate Platform to Detect Biomarkers in the Skin |
| 260 | |b University of Huddersfield Press, |c 2022-11-01T00:00:00Z. | ||
| 500 | |a 10.5920/bjpharm.1164 | ||
| 500 | |a 2058-8356 | ||
| 520 | |a This research aimed to integrate an established signal quantification technique with immunocapture microneedle arrays (MNAs) to improve biomarker-based disease diagnostics in the skin. In this miniaturized sandwich enzyme-linked immunosorbent assay (ELISA) platform, the capture antibody was immobilised onto the surface of microneedles which, when inserted superficially into porcine skin, captured endogenous porcine immunoglobulin G (IgG) intradermally. Signal development was achieved by incubating the microneedles into-phenylenediamine (OPD) solution in a 384-well microplate and measuring the absorbance in a microplate spectrophotometer at 450 nm. This technique allows for rapid biomarker detection with high-throughput processing. Immunocapture microneedle devices such as these can be easily adapted for targeting different biomarkers or multiplexed leaving plenty of scope for future work and assay optimisation. | ||
| 546 | |a EN | ||
| 690 | |a microneedle array (mna) | ||
| 690 | |a point-of-care testing (poct) | ||
| 690 | |a diagnostics | ||
| 690 | |a elisa | ||
| 690 | |a Pharmacy and materia medica | ||
| 690 | |a RS1-441 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n British Journal of Pharmacy, Vol 7, Iss 2 (2022) | |
| 787 | 0 | |n https://www.bjpharm.org.uk/article/id/1164/ | |
| 787 | 0 | |n https://doaj.org/toc/2058-8356 | |
| 856 | 4 | 1 | |u https://doaj.org/article/54d0a1a14979443d82c97a6870d82da0 |z Connect to this object online. |