Does resveratrol affect prepared sperm parameters and chromatin quality in normozoospermic and asthenozoospermic patients before and after freezing? A lab trial study

• Main Authors: Masoomeh Mohammadzadeh (Author), Mohammad Ali khalili (Author), Vahid Ramezani (Author), Hamed Hamishehkar (Author), Marvast Laleh Dehghan (Author), Esmat Mangoli (Author), Mahya Rajabi (Author), Zhima Akhavan Sales (Author), Ali Reza Talebi (Author)
• Format: Book
• Published: Shahid Sadoughi University of Medical Sciences, 2020-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 2476-3772; 10.18502/ijrm.v13i9.7670
• Keywords: Gynecology and obstetrics; RG1-991; Reproduction; QH471-489
• Source: International Journal of Reproductive BioMedicine, Vol 18, Pp 755-764 (2020)

Background: Previous studies have examined the effect of resveratrol as a potent antioxidant for free radicals in semen. While, the prepared spermatozoa are more affected by ROS factors due to centrifugation and incubation. Objective: To evaluate the RSV's effects on the prepared sperm parameters and chromatin quality in both normozoospermic and asthenozoospermic cases before and after freezing. Materials and Methods: The sample of 10 normozoospermic and asthenozoospermic men was prepared through the swim-up method. The groups were then divided into two samples of control and experimental (exposure to 30 µmol/l of RSV) to evaluate and compare the sperm parameters and chromatin quality before and after freezing. Results: The motility and viability of spermatozoa were seen to be significantly different before and after freezing separately in the control and treatment samples of the groups (p ≤ 0.001 and p = 0.001, respectively). However, the stated difference between the control and treatment samples of normozoospermic and asthenozoospermic patients were not significant (p > 0.05). In addition, the sperm morphology and chromatin quality were not significantly different between the two samples of each group; nonetheless, chromatin quality of the treated sample was better than that of the control before and after freezing. Conclusion: Despite the protective effects of RSV on the semen samples, RSV cannot affect significantly the prepared sperms parameters and chromatin quality in normozoospermic and asthenozoospermic patients.Key words: Resveratrol, Chromatin, Motility, Spermatozoa, Freeze.