Purification of bacteriocins using size-exclusion chromatography
The bacteriocin purification involves following main steps. a). Extraction of cell-free-supernatant of bacteria. b). Ammonium sulfate precipitation. c). Dialysis. d). Diafiltration using PVP and e). Size-exclusion chromatography. However, depending on the nature of work, the compound could be furthe...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Book |
| Published: |
Bangladesh Pharmacological Society,
2016-03-01T00:00:00Z.
|
| Subjects: | |
| Online Access: | Connect to this object online. |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | The bacteriocin purification involves following main steps. a). Extraction of cell-free-supernatant of bacteria. b). Ammonium sulfate precipitation. c). Dialysis. d). Diafiltration using PVP and e). Size-exclusion chromatography. However, depending on the nature of work, the compound could be further analyzed by reverse-phase HPLC, NMR, mass spectrometry and sequencing. Video Clips of Methodology: Part 1: 4 min 52 sec Full Screen Alternate Part 2: 1 min 47 sec Full Screen Alternate |
|---|---|
| Item Description: | 10.3329/bjp.v11i2.25862 1991-0088 |