Effects of some compounds on the A-Amylase Activity Purified From Abena-24 Wheat Flour Treated by TriboliumConfusum
a-Ainylase was purified from Abena-24 wheat flour treated by Triboliumconfusuminsect using heat treatment, ammonium sulphateprecipitation, dialysis, and anion exchange chromatography.<br /> Three a-amylase isoenzymes were isolated (I, II and III) with elution volume of 90, 170 and 220 ml respe...
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College of Education for Pure Sciences,
2005-06-01T00:00:00Z.
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| Summary: | a-Ainylase was purified from Abena-24 wheat flour treated by Triboliumconfusuminsect using heat treatment, ammonium sulphateprecipitation, dialysis, and anion exchange chromatography.<br /> Three a-amylase isoenzymes were isolated (I, II and III) with elution volume of 90, 170 and 220 ml respectively, with specific activity of 73000, 185818 and 462333 mu/mg protein, respectively compared<br /> with the crude enzyme. Many compounds showed different effects on the a-amylase isoenzymes activity. Pheriyl .hydrazine, EDTA and HC1 showed an inhibitory effect between (70-80%), (49-53.5%) and (46.4-67.5%) respectively. Using heavy metales , it was observed that Cu+2 caused an inhibitory effect between (32.8-42,2%>), while Fe+2 caused an inhibitory effect between (26.3-53.5%). On the other hand, no significant inhibitory effect was shown with iodoacetamide and tristearin. Chloride ion and calcium ion showed an activitory effects on the a-amylase isoenzymes 1, II and III ranging between. (27-45.4%) for CI" and (15-82%) for Ca+J |
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| Item Description: | 1812-125X 2664-2530 10.33899/edusj.2005.81314 |