The ability of IgY to recognize surface proteins of Streptococcus mutans

• Main Authors: Basri A. Gani (Author), Santi Chismirina (Author), Zinatul Hayati (Author), Endang Winiati B (Author), Boy M. Bachtiar (Author), I. Wayan T. Wibawan (Author)
• Format: Book
• Published: Universitas Airlangga, 2009-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 1978-3728; 2442-9740; 10.20473/j.djmkg.v42.i4.p189-193
• Keywords: IgY; Streptococcus mutans; adhesion; surface proteins; and dental caries; Dentistry; RK1-715
• Source: Dental Journal, Vol 42, Iss 4, Pp 189-193 (2009)

<span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Background: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Streptococcus mutans are gram positive bacteria classified into viridians group, and have a role in pathogenesis </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>of dental caries. It's adhesion to the tooth surface is mediated by cell surface proteins, which interact with specific receptor located </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>in tooth pellicle. Glucan binding protein, Glukosyltransferase, and antigen I/II are basic proteins of S. mutans, which have a role in </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>initiating the interaction. A previous study showed that chicken's IgY can interfere the interaction. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Purpose</strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>: The objective of this </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>study was to assess the ability of IgY in recognizing the surface molecule of Streptococcus mutans expressed by various serotypes </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>(c, d, e, f) and a strain derived from IPB, Bogor. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Method</strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>: Western blot was used as a method to determine such capability. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Result</strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>: </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The result showed that IgY has a potency to recognize antigen I/II, but not the other proteins on the cell surface of all bacteria tested. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Conclusion: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The ability of IgY to bind the surface protein, antigen I/II, indicates that this avian antibody could be used as a candidate </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>for anti-adhesion in preventing dental caries.</em></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span>