Klebsiella pneumoniae enolase-like membrane protein interacts with human plasminogen

• Main Authors: Paweł Serek (Author), Łukasz Lewandowski (Author), Bartłomiej Dudek (Author), Jadwiga Pietkiewicz (Author), Katarzyna Jermakow (Author), Katarzyna Kapczyńska (Author), Eva Krzyżewska (Author), Iwona Bednarz-Misa (Author)
• Format: Book
• Published: Elsevier, 2021-08-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 1438-4221; 10.1016/j.ijmm.2021.151518
• Keywords: Enolase-like protein; Klebsiella pneumoniae; Outer membrane proteins; Plasminogen; Virulence factor; Microbiology; QR1-502; Other systems of medicine; RZ201-999
• Source: International Journal of Medical Microbiology, Vol 311, Iss 6, Pp 151518- (2021)

Many models assessing the risk of sepsis utilize the knowledge of the constituents of the plasminogen system, as it is proven that some species of bacteria can activate plasminogen, as a result of interactions with bacterial outer membrane proteins. However, much is yet to be discovered about this interaction since there is little information regarding some bacterial species. This study is aimed to check if Klebsiella pneumoniae, one of the major factors of nosocomial pneumonia and a factor for severe sepsis, has the ability to bind to human plasminogen. The strain used in this study, PCM 2713, acted as a typical representative of the species. With use of various methods, including: electron microscopy, 2-dimensional electrophoresis, immunoblotting and peptide fragmentation fingerprinting, it is shown that Klebsiella pneumoniae binds to human plasminogen, among others, due to plasminogen-bacterial enolase-like protein interaction, occurring on the outer membrane of the bacterium. Moreover, the study reveals, that other proteins, such as: phosphoglucomutase, and phosphoenolpyruvate carboxykinase act as putative plasminogen-binding factors. These information may virtually act as a foundation for future studies investigating: the: pathogenicity of Klebsiella pneumoniae and means for prevention from the outcomes of Klebsiella-derived sepsis.