The in vitro assessment of anti proliferation activity of crude diethyl ether extract of Dendrophthoe species against to myeloma culture cell
<span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Herb medicine have an active substances that can dissolved on polar, semi polar and non polar liquid extract. The methanol </em><span style="fo...
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Universitas Airlangga,
2008-03-01T00:00:00Z.
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| Summary: | <span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Herb medicine have an active substances that can dissolved on polar, semi polar and non polar liquid extract. The methanol </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>and ethyl acetate as a polar and semi polar extract liquid were used to study of herb medicine such as Dendrophthoe species. Diethyl </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>ether as a non polar liquid extract was never use to study the Dendrophthoe species. The aim of this study was to investigate the anti </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>proliferation activity of Dendrophthoe species to myeloma culture cells after extracted by non-polar extract solution (diethyl ether). </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The post test only control group design was used for this research. A thirty six of microtiterplates wells were used for myeloma culture </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>cells in RPMI medium. The wells were devided placing in two groups: treatment groups and controls groups. Each three wells of </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>six treatment subgroups added with 100 </em><span style="font-family: Symbol; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;">µ<span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>l of 1.1; 5.5; 11; 22; 33 and 44 </em><span style="font-family: Symbol; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;">µ<span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>g/ml crude diethyl ether extract series. A RPMI solution at </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>similar method and volume were used as control substances. The cells were assessed by inverted microscope in 200x magnified two </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>days after. The cells were quantified analyzed for anti proliferation activity by using 1:1 methylene blue solution. The results showed </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>that started from 11.0 </em><span style="font-family: Symbol; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;">µ<span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>g/ml of crude diethyl ether extract of Dendrophthoe species have been anti proliferation abilities of myeloma </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>culture cells (p</em><span style="font-family: Symbol; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><<span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>0.05). In conclusion, actives substances of Dendrophthoe species where dissolved in non polar liquid as diethyl ether </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>has anti proliferation activities to cancer cell in vitro.</em></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span> |
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| Item Description: | 1978-3728 2442-9740 10.20473/j.djmkg.v41.i1.p5-9 |