<i>Trueperella pyogenes</i> Isolates from Livestock and European Bison (<i>Bison bonasus</i>) as a Reservoir of Tetracycline Resistance Determinants
Determinants of tetracycline resistance in <i>Trueperella pyogenes</i> are still poorly known. In this study, resistance to tetracycline was investigated in 114 <i>T. pyogenes</i> isolates from livestock and European bison. Tetracycline minimum inhibitory concentration (MIC)...
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| Main Authors: | , , , , , , , , |
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| Format: | Book |
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MDPI AG,
2021-04-01T00:00:00Z.
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| Summary: | Determinants of tetracycline resistance in <i>Trueperella pyogenes</i> are still poorly known. In this study, resistance to tetracycline was investigated in 114 <i>T. pyogenes</i> isolates from livestock and European bison. Tetracycline minimum inhibitory concentration (MIC) was evaluated by a microdilution method, and tetracycline resistance genes were detected by PCR. To determine variants of <i>tetW</i> and their linkage with mobile elements, sequencing analysis was performed. Among the studied isolates, 43.0% were tetracycline resistant (MIC ≥ 8 µg/mL). The highest MIC<sub>90</sub> of tetracycline (32 µg/mL) was noted in bovine and European bison isolates. The most prevalent determinant of tetracycline resistance was <i>tetW</i> (in 40.4% of isolates), while <i>tetA(33)</i> was detected only in 8.8% of isolates. Four variants of <i>tetW</i> (<i>tetW-1</i>, <i>tetW-2</i>, <i>tetW-3</i>, <i>tetW-4</i>) were recognized. The <i>tetW-3</i> variant was the most frequent and was linked to the ATE-1 transposon. The <i>tetW-</i>2 variant, found in a swine isolate, was not previously reported in <i>T. pyogenes</i>. This is the first report on determinants of tetracycline resistance in <i>T. pyogenes</i> isolates from European bison. These findings highlight that wild animals, including wild ruminants not treated with antimicrobials, can be a reservoir of tetracycline-resistant bacteria carrying resistance determinants, which may be easily spread among pathogenic and environmental microorganisms. |
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| Item Description: | 10.3390/antibiotics10040380 2079-6382 |