Persistence of Antibiotic-Resistant <i>Escherichia coli</i> Strains Belonging to the B2 Phylogroup in Municipal Wastewater under Aerobic Conditions
<i>Escherichia coli</i> is classified into four major phylogenetic groups (A, B1, B2, and D) that are associated with antibiotic resistance genes. Although antibiotic-resistant <i>E. coli</i> is commonly detected in municipal wastewater, little is known about the relationship...
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2022-02-01T00:00:00Z.
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| LEADER | 00000 am a22000003u 4500 | ||
|---|---|---|---|
| 001 | doaj_5e1aa497a8bc4e7b98c054742a65a6a2 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Hui Xie |e author |
| 700 | 1 | 0 | |a Yoshitoshi Ogura |e author |
| 700 | 1 | 0 | |a Yoshihiro Suzuki |e author |
| 245 | 0 | 0 | |a Persistence of Antibiotic-Resistant <i>Escherichia coli</i> Strains Belonging to the B2 Phylogroup in Municipal Wastewater under Aerobic Conditions |
| 260 | |b MDPI AG, |c 2022-02-01T00:00:00Z. | ||
| 500 | |a 10.3390/antibiotics11020202 | ||
| 500 | |a 2079-6382 | ||
| 520 | |a <i>Escherichia coli</i> is classified into four major phylogenetic groups (A, B1, B2, and D) that are associated with antibiotic resistance genes. Although antibiotic-resistant <i>E. coli</i> is commonly detected in municipal wastewater, little is known about the relationship between the phylogenetic groups and antibiotic-resistant <i>E. coli</i> in wastewater. In this study, the survival of <i>E. coli</i> in wastewater and the changes to the relationships between each phylogroup and the antibiotic-resistant profiles of <i>E. coli</i> isolates from wastewater were investigated under aerobic conditions for 14 days. The isolates were classified into the phylogroups A, B1, B2, and D or others by multiplex PCR. In addition, the susceptibility of the isolates to 11 antibiotics was assessed with the minimum inhibitory concentration (MIC) assay. While <i>E. coli</i> counts decreased in the wastewater with time under aerobic conditions, the prevalence of phylogroup B2 had increased to 73% on day 14. Furthermore, the MIC assay revealed that the abundance of antibiotic-resistant <i>E. coli</i> also increased on day 14. After batch-mixing the experiments under aerobic conditions, the surviving antibiotic-resistant <i>E. coli</i> included mainly multidrug-resistant and beta-lactamase-producing isolates belonging to phylogroup B2. These results suggest that the phylogroup B2 isolates that have acquired antibiotic resistance had a high survivability in the treated wastewater. | ||
| 546 | |a EN | ||
| 690 | |a <i>Escherichia coli</i> | ||
| 690 | |a antibiotic resistant | ||
| 690 | |a phylogroups | ||
| 690 | |a municipal wastewater | ||
| 690 | |a Therapeutics. Pharmacology | ||
| 690 | |a RM1-950 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Antibiotics, Vol 11, Iss 2, p 202 (2022) | |
| 787 | 0 | |n https://www.mdpi.com/2079-6382/11/2/202 | |
| 787 | 0 | |n https://doaj.org/toc/2079-6382 | |
| 856 | 4 | 1 | |u https://doaj.org/article/5e1aa497a8bc4e7b98c054742a65a6a2 |z Connect to this object online. |