Persistence of Antibiotic-Resistant <i>Escherichia coli</i> Strains Belonging to the B2 Phylogroup in Municipal Wastewater under Aerobic Conditions

<i>Escherichia coli</i> is classified into four major phylogenetic groups (A, B1, B2, and D) that are associated with antibiotic resistance genes. Although antibiotic-resistant <i>E. coli</i> is commonly detected in municipal wastewater, little is known about the relationship...

Full description

Saved in:
Bibliographic Details
Main Authors: Hui Xie (Author), Yoshitoshi Ogura (Author), Yoshihiro Suzuki (Author)
Format: Book
Published: MDPI AG, 2022-02-01T00:00:00Z.
Subjects:
Online Access:Connect to this object online.
Tags: Add Tag
No Tags, Be the first to tag this record!

MARC

LEADER 00000 am a22000003u 4500
001 doaj_5e1aa497a8bc4e7b98c054742a65a6a2
042 |a dc 
100 1 0 |a Hui Xie  |e author 
700 1 0 |a Yoshitoshi Ogura  |e author 
700 1 0 |a Yoshihiro Suzuki  |e author 
245 0 0 |a Persistence of Antibiotic-Resistant <i>Escherichia coli</i> Strains Belonging to the B2 Phylogroup in Municipal Wastewater under Aerobic Conditions 
260 |b MDPI AG,   |c 2022-02-01T00:00:00Z. 
500 |a 10.3390/antibiotics11020202 
500 |a 2079-6382 
520 |a <i>Escherichia coli</i> is classified into four major phylogenetic groups (A, B1, B2, and D) that are associated with antibiotic resistance genes. Although antibiotic-resistant <i>E. coli</i> is commonly detected in municipal wastewater, little is known about the relationship between the phylogenetic groups and antibiotic-resistant <i>E. coli</i> in wastewater. In this study, the survival of <i>E. coli</i> in wastewater and the changes to the relationships between each phylogroup and the antibiotic-resistant profiles of <i>E. coli</i> isolates from wastewater were investigated under aerobic conditions for 14 days. The isolates were classified into the phylogroups A, B1, B2, and D or others by multiplex PCR. In addition, the susceptibility of the isolates to 11 antibiotics was assessed with the minimum inhibitory concentration (MIC) assay. While <i>E. coli</i> counts decreased in the wastewater with time under aerobic conditions, the prevalence of phylogroup B2 had increased to 73% on day 14. Furthermore, the MIC assay revealed that the abundance of antibiotic-resistant <i>E. coli</i> also increased on day 14. After batch-mixing the experiments under aerobic conditions, the surviving antibiotic-resistant <i>E. coli</i> included mainly multidrug-resistant and beta-lactamase-producing isolates belonging to phylogroup B2. These results suggest that the phylogroup B2 isolates that have acquired antibiotic resistance had a high survivability in the treated wastewater. 
546 |a EN 
690 |a <i>Escherichia coli</i> 
690 |a antibiotic resistant 
690 |a phylogroups 
690 |a municipal wastewater 
690 |a Therapeutics. Pharmacology 
690 |a RM1-950 
655 7 |a article  |2 local 
786 0 |n Antibiotics, Vol 11, Iss 2, p 202 (2022) 
787 0 |n https://www.mdpi.com/2079-6382/11/2/202 
787 0 |n https://doaj.org/toc/2079-6382 
856 4 1 |u https://doaj.org/article/5e1aa497a8bc4e7b98c054742a65a6a2  |z Connect to this object online.