A Comparative Biochemical Study of Proteins Profile in Iraqi Children and Adolescent with ?-Thalassemia

• Main Authors: Ali M. Malik (Author), Emad M. Malik (Author), Nawal MJ Al-Shammaa (Author), Zeinab M. Al-Rubaei (Author)
• Format: Book
• Published: College of Pharmacy University of Baghdad, 2017-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 10.31351/vol19iss2pp19-23; 2521-3512; 1683-3597
• Keywords: Pharmacy and materia medica; RS1-441
• Source: Iraqi Journal of Pharmaceutical Sciences, Vol 19, Iss 2 (2017)

The aim of the present research is to study different protein fractions in sera of children and adolescent with β â€"thalassemia major and minor and to compare the results with that of healthy control.One hundred fifty children and adolescents were enrolled in this study,including 50 patients with β- thalassemia major , 50 patients with β- thalassemia minor as pathological control group and another apparently 50 healthy individuals as a control group. The age of all studied groups ranged from (4-18)years.Total protein, albumin and immunoglobulins were estimated in sera of all subjects. A Significant decrease was found in the total protein and albumin levels in sera of both major and minor thalassemic patients compared to normal groups. A Significant increase in immunoglobulin levels (IgG, IgM and IgA) was found in the sera of major and minor β-thalassemia patients compared to control.Different protein parts in sera of all subjects were detected using cellulose acetate electrophoresis.The results revealed significant reduction in β- globulin fractions in β- thalassemia major patients compared to the normal and pathological control groups. Significant elevations in γ- globulins fractions were observed in both major β- thalassemia and minor β- thalassemia as compared to normal control group. As a Conclusion the alteration in some protein parts occurred which is more obvious in major thalassemia patients compared to the normal and pathological control groups. Key words: Protein Parts , Elecrtrophoresis , β-Thalassemia