Anticarcinogenic effect of alpha-lipoic acid and <i>Jatropha gossypifolia</i> leaves against cypermethrin-induced genotoxicity in rats

• Main Authors: Opeyemi O. Faokunla (Author), Oluseyi A. Akinloye (Author), Regina N. Ugbaja (Author), Abideen I. Adeogun (Author)
• Format: Book
• Published: AOSIS, 2018-02-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 2519-559X; 2616-4809; 10.4102/jomped.v2i1.45
• Keywords: p53; cypermethrin; Jatropha gossypifolia; alpha-lipoic acid; genotoxicity; Botany; QK1-989; Pharmacy and materia medica; RS1-441
• Source: Journal of Medicinal Plants for Economic Development, Vol 2, Iss 1, Pp e1-e5 (2018)

Background: Cypermethrin (Cyp), a synthetic pyrethroid developed for agricultural and public health applications, has become harmful to the environment and mammalian life owing to indiscriminate use. Aim: This study aimed to investigate the protective effect of the methanolic extract of Jatropha gossypifolia leaves against Cyp-induced genotoxicity in rats using alpha-lipoic acid (α-LA) as standard drug. Method: Fifty-six male Wistar rats, assigned to eight groups of seven animals each, were treated orally for 28 days as follows - Group 1: only corn oil (Control); Group 2: 20 mg/kg body weight (b.wt) of Cyp; Groups 3, 4 and 5: Cyp + 50 mg/kg b.wt of J. gossypifolia, Cyp + 100 mg/kg b.wt of J. gossypifolia and Cyp + 50 mg/kg b.wt of α-LA, respectively; Groups 6, 7 and 8: J. gossypifolia (50 mg/kg b.wt), J. gossypifolia (100 mg/kg b.wt) and α-LA (50 mg/kg b.wt), respectively. The P53 was assayed for using the plasma, while the micronucleus assay was carried out on the bone marrow. Results: Results showed that exposure to Cyp significantly increased the number of micronucleated polychromatic erythrocytes (86.70%) as well as p53 concentration in both the liver and plasma (200.00% and 125.53%) compared to the control. Co-administration with methanolic extract of J. gossypifolia leaves significantly reversed these alterations in a dose-dependent manner. Conclusion: The methanolic extract of J. gossypifolia leaves has the potential to ameliorate Cyp-induced genotoxicity in rats, and also, the toxicological evaluations of the plant extract and α-LA showed that it is relatively safe for use at the doses investigated.