Rapid Detection of Carbapenemases Using NG-Test^® CARBA 5 in Positive Blood Cultures: A Diagnostic Test Study

• Main Authors: Diana Munguia-Ramos (Author), Luis Fernando Xancal-Salvador (Author), Verónica Esteban-Kenel (Author), Narciso Ortiz-Conchi (Author), Ricardo Antonio Jaimes-Aquino (Author), Miguel Mendoza-Rojas (Author), Axel Cervantes-Sánchez (Author), Steven Méndez-Ramos (Author), Hector Orlando Rivera-Villegas (Author), Sandra Rajme-Lopez (Author), Karla Maria Tamez-Torres (Author), Carla Marina Roman-Montes (Author), Areli Martínez-Gamboa (Author), Miriam Bobadilla del-Valle (Author), Jose Sifuentes-Osornio (Author), Alfredo Ponce- (Author), Maria Fernanda Gonzalez-Lara (Author), Bernardo Alfonso Martinez-Guerra (Author)
• Format: Book
• Published: MDPI AG, 2024-11-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Background: Infections due to carbapenem-resistant Gram-negative bacteria are emerging as an important challenge in health-care settings and a growing concern worldwide. Lateral flow immunoassay NG-Test^® CARBA 5 can detect the five most reported carbapenemases (KPC, OXA-48-like, VIM, IMP, and NDM). Direct testing of positive blood cultures could reduce time to detection. This study aims to validate and report on the diagnostic yield of a novel method for carbapenemase detection in positive blood culture vials using NG-Test^® CARBA 5. Methods: We implemented an investigator-developed method for the direct testing of positive blood cultures using NG-Test^® CARBA 5. We compared results between genotypic, phenotypic, and direct NG-Test^® CARBA 5 in blood. Results: A total of 32 isolates were tested (21 Enterobacterales and 11 <i>Pseudomonas aeruginosa</i>). Genotypic testing detected 23 carbapenemases. When comparing the results of NG-Test^® CARBA 5 in blood with genotypic testing, agreement was observed in 31/32 (97%) tests. The sensitivity, specificity, positive predictive value, and negative predictive value of the NG-Test^® CARBA 5 in blood were 93%, 100%, 100%, and 94%, respectively. Conclusions: Our method using NG-Test^® CARBA 5 directly in blood culture samples presented an excellent diagnostic yield when compared to genotypic profiling and permits an accurate detection of carbapenemases.

Item Description

10.3390/antibiotics13111105
2079-6382