Characterization of Extended-Spectrum Beta-Lactamase-Producing <i>Escherichia coli</i> in Diarrhoeal Faeces from 0 to 5-Year-Old Children Attending Public Hospitals in Franceville, Gabon

• Main Authors: Anicet-Clotaire Dikoumba (Author), Pierre Philippe Mbehang Nguema (Author), Leresche Even Doneilly Oyaba Yinda (Author), Romeo Wenceslas Lendamba (Author), Jean Constan Obague Mbeang (Author), Guy Roger Ndong Atome (Author), Christophe Roland Zinga Koumba (Author), Sylvain Godreuil (Author), Richard Onanga (Author)
• Format: Book
• Published: MDPI AG, 2024-11-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Background: In Gabon, studies on the characterization of extended-spectrum beta-lactamase-producing <i>Escherichia coli</i> in young children with diarrhoea are almost nonexistent. The objective was to evaluate the prevalence of antibiotic resistance to extended-spectrum beta-lactamase-producing <i>Escherichia coli</i> in children at public hospitals in Franceville, Gabon. Methods: Seventy diarrhoea faecal samples were collected from children aged 0-5 years. The culture and isolation of colonies were carried out on MacConkey agar. The colonies were identified using VITEK 2. The determination of the extended-spectrum beta-lactamase's profiles was accomplished using the double disk method. The identification of phylogroups and pathotypes was performed by PCR. Identification of the ESBL genes was performed by sequencing. Results: A total of 26 strains of <i>Escherichia coli</i> (33.0%) were identified from 78 bacterial isolates. Twenty (77.0%) <i>Escherichia coli</i> strains carried extended-spectrum beta-lactamases bla_CTX-M-15 and 5.0% carried bla_SHV-12 subtypes. Phylogroup D (62.0%) was predominant, followed by B1 (12.0%), B2 (8.0%) and E (4.0%). The bacterial pathogens causing diarrhoea were enterohemorrhagic <i>E. coli</i> (12.0%), typical enteropathogenic <i>Escherichia coli</i> (8.0%), atypical enteropathogenic <i>Escherichia coli</i> (4.0%), Enteroaggregative <i>Escherichia coli</i> (4.0%) and enteroinvasive <i>E. coli</i> (4.0%). Conclusions: This study showed a high prevalence of extended-spectrum beta-lactamase, <i>Escherichia coli</i> of phylogroup D and pathotype enterohemorrhagic <i>Escherichia coli</i> in children under 5 years old in public hospitals in Franceville, most probably due to the misuse or inappropriate consumption of beta-lactams.

Item Description

10.3390/antibiotics13111059
2079-6382