Screening Tests for the Interaction of <i>Rubus idaeus</i> and <i>Rubus occidentalis</i> Extracts with Antibiotics against Gram-Positive and Gram-Negative Human Pathogens

WHO (World Health Organization) reports from recent years warn about the growing number of antibiotic-resistant bacterial strains. Therefore, there is an urgent need to constantly search for new substances effective in the fight against microorganisms. Plants are a rich source of chemical compounds...

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Main Authors: Rafał Hałasa (Author), Urszula Mizerska (Author), Marta Kula (Author), Mirosława Krauze-Baranowska (Author)
Format: Book
Published: MDPI AG, 2024-07-01T00:00:00Z.
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001 doaj_619cbdbb4cce4d5a97aab9b0d1a534c1
042 |a dc 
100 1 0 |a Rafał Hałasa  |e author 
700 1 0 |a Urszula Mizerska  |e author 
700 1 0 |a Marta Kula  |e author 
700 1 0 |a Mirosława Krauze-Baranowska  |e author 
245 0 0 |a Screening Tests for the Interaction of <i>Rubus idaeus</i> and <i>Rubus occidentalis</i> Extracts with Antibiotics against Gram-Positive and Gram-Negative Human Pathogens 
260 |b MDPI AG,   |c 2024-07-01T00:00:00Z. 
500 |a 10.3390/antibiotics13070653 
500 |a 2079-6382 
520 |a WHO (World Health Organization) reports from recent years warn about the growing number of antibiotic-resistant bacterial strains. Therefore, there is an urgent need to constantly search for new substances effective in the fight against microorganisms. Plants are a rich source of chemical compounds with antibacterial properties. These compounds, classified as secondary metabolites, may act independently or support the action of currently used antibiotics. Due to the large number of metabolites isolated from the plant kingdom and new plant species being studied, there is a need to develop new strategies/techniques or modifications of currently applied methods that can be used to select plant extracts or chemical compounds isolated from them that enter into positive, synergistic interactions with currently used antibiotics. One such method is the dual-disk synergy test (DDST). It involves the diffusion of active compounds in the agar environment and influencing the growth of microorganisms grown on it. The method was used to assess the interaction of extracts from the fruit and shoots of some cultivated varieties of <i>Rubus idaeus</i> and <i>Rubus occidentalis</i> with selected antibiotics. The research was conducted on strains of bacteria pathogenic to humans, including <i>Staphylococcus aureus</i>, <i>Corynebacterium diphtheriae</i>, <i>Escherichia coli</i>, <i>Pseudomonas aeruginosa</i>, <i>Helicobacter pylori</i>, and <i>Candida albicans</i>, showing synergy, antagonism, or lack of interaction of the tested substances-plant extract and antibiotic. As a result, it was found that the diffusion method is useful in screening tests to assess the impact of antibiotic-herbal substance interactions on Gram-positive and Gram-negative microorganisms. 
546 |a EN 
690 |a additivity 
690 |a antagonisms 
690 |a antibiotics 
690 |a double-disk synergy test 
690 |a extracts 
690 |a <i>Rubus</i> sp. 
690 |a Therapeutics. Pharmacology 
690 |a RM1-950 
655 7 |a article  |2 local 
786 0 |n Antibiotics, Vol 13, Iss 7, p 653 (2024) 
787 0 |n https://www.mdpi.com/2079-6382/13/7/653 
787 0 |n https://doaj.org/toc/2079-6382 
856 4 1 |u https://doaj.org/article/619cbdbb4cce4d5a97aab9b0d1a534c1  |z Connect to this object online.