The molecular determination of <i>emm</i> genotypes in non-group a beta-hemolytic streptococci isolated from clinical samples

• Main Authors: Suna Kızılyıldırım (Author), Cansu Önlen Güneri (Author), Fatih Köksal (Author)
• Format: Book
• Published: National Scientific Medical Center, 2021-08-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

<b>Objective: </b>M protein is an important marker in epidemiological and phylogenetic surveillance of Beta-Hemolytic Streptococci.<br /> The aim of this study is to determine <i>emm</i> types and their distribution among the GCS/GGS strains isolated from clinical samples.<br /> <b>Material and methods:</b> The study includes 98 β-hemolytic streptococcus strains isolated from clinical samples in Çukurova University/Balcalı Hospital. 67 of these isolates, defined as serologic, of which 56 were identified as Streptococcus dysgalactiae subsp. equisimilis, 8 as <i>S. anginosus </i>and 3 as <i>S.equi subsp.zooepedimicus, </i>have been included in the study. These strains were also confirmed by the Vitek 2GP-ID system. These isolates were confirmed by the polymerase chain reaction (PCR) method with primers based on groESL sequences. PCR-sequence analysis method developed by the Centers for Disease Control (CDC) was used for typing according to <i>emm</i> polymorphism.<br /> <b>Results:</b> 56/67 (83.6%) isolates in which <i>emm-PCR</i> was determined as positive were typed by sequence analysis. The findings were identified using the CDC database, and the most common type was determined to be stG485 (51.85%), stG643 (12.96%) and stG6 (9.25%).<br /> <b>Conclusion:</b> Consequently, GCS/GGS should be treated more seriously in our country as in the whole world.

Item Description

1812-2892
2313-1519
10.23950/jcmk/11045