Methylglyoxal Augments Angiotensin II-Induced Contraction in Rat Isolated Carotid Artery
Abstract.: Methylglyoxal (MGO), a metabolite of glucose, accumulates in vascular tissues of a hypertensive animal. In the present study, we examined the effect of MGO on angiotensin (Ang) II-induced contraction of rat carotid artery. Treatment of carotid artery with MGO (420 μM, 30 min) significantl...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Book |
| Published: |
Elsevier,
2010-01-01T00:00:00Z.
|
| Subjects: | |
| Online Access: | Connect to this object online. |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
MARC
| LEADER | 00000 am a22000003u 4500 | ||
|---|---|---|---|
| 001 | doaj_628acbc846034b27b6163bc876bbf5d0 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Masashi Mukohda |e author |
| 700 | 1 | 0 | |a Hideyuki Yamawaki |e author |
| 700 | 1 | 0 | |a Muneyoshi Okada |e author |
| 700 | 1 | 0 | |a Yukio Hara |e author |
| 245 | 0 | 0 | |a Methylglyoxal Augments Angiotensin II-Induced Contraction in Rat Isolated Carotid Artery |
| 260 | |b Elsevier, |c 2010-01-01T00:00:00Z. | ||
| 500 | |a 1347-8613 | ||
| 500 | |a 10.1254/jphs.10206FP | ||
| 520 | |a Abstract.: Methylglyoxal (MGO), a metabolite of glucose, accumulates in vascular tissues of a hypertensive animal. In the present study, we examined the effect of MGO on angiotensin (Ang) II-induced contraction of rat carotid artery. Treatment of carotid artery with MGO (420 μM, 30 min) significantly augmented Ang II (0.1 to 30 nM)-induced concentration-dependent contraction. The effect was abolished by the removal of endothelium. BQ-123 (1, 5 μM), an endothelin A- receptor blocker, had no effect on the MGO-induced enhancement of Ang II-induced contraction. AL8810 (1 μM), a prostaglandin F2α-receptor blocker, or SQ29548 (1 μM), a thromboxane A2- receptor blocker, was also ineffective. However, tempol (10 μM), a superoxide scavenger, and catalase (5000 U/mL), which metabolizes hydrogen peroxide to water, significantly prevented the effect of MGO. Combined MGO and Ang II treatment increased reactive oxygen species (ROS) production. Apocynin (10 μM) or gp91ds-tat (3 μM), an inhibitor of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, significantly prevented the effect of MGO. Gp91ds-tat or an Ang II type 1-receptor (AT1R) blocker, losartan (10 μM), prevented the MGO-mediated increased ROS production. The present study revealed that MGO augments Ang II-induced contraction by increasing AT1R-mediated NADPH oxidase-derived superoxide and hydrogen peroxide production in endothelium of rat carotid artery. Keywords:: glucose metabolite, vascular endothelium, angiotensin, reactive oxygen species, smooth muscle contractility | ||
| 546 | |a EN | ||
| 690 | |a Therapeutics. Pharmacology | ||
| 690 | |a RM1-950 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Journal of Pharmacological Sciences, Vol 114, Iss 4, Pp 390-398 (2010) | |
| 787 | 0 | |n http://www.sciencedirect.com/science/article/pii/S1347861319308175 | |
| 787 | 0 | |n https://doaj.org/toc/1347-8613 | |
| 856 | 4 | 1 | |u https://doaj.org/article/628acbc846034b27b6163bc876bbf5d0 |z Connect to this object online. |