The Effects of Platelet-Derived Growth Factor-BB on Bone Marrow Stromal Cell-Mediated Vascularized Bone Regeneration
Regenerative medicine for bone tissue mainly depends on efficient recruitment of endogenous or transplanted stem cells to guide bone regeneration. Platelet-derived growth factor (PDGF) is a functional factor that has been widely used in tissue regeneration and repair. However, the short half-life of...
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| Format: | Book |
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Hindawi Limited,
2018-01-01T00:00:00Z.
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| LEADER | 00000 am a22000003u 4500 | ||
|---|---|---|---|
| 001 | doaj_62d6f4bdeb84483c8b36dfb45ddce74b | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Maolin Zhang |e author |
| 700 | 1 | 0 | |a Wenwen Yu |e author |
| 700 | 1 | 0 | |a Kunimichi Niibe |e author |
| 700 | 1 | 0 | |a Wenjie Zhang |e author |
| 700 | 1 | 0 | |a Hiroshi Egusa |e author |
| 700 | 1 | 0 | |a Tingting Tang |e author |
| 700 | 1 | 0 | |a Xinquan Jiang |e author |
| 245 | 0 | 0 | |a The Effects of Platelet-Derived Growth Factor-BB on Bone Marrow Stromal Cell-Mediated Vascularized Bone Regeneration |
| 260 | |b Hindawi Limited, |c 2018-01-01T00:00:00Z. | ||
| 500 | |a 1687-966X | ||
| 500 | |a 1687-9678 | ||
| 500 | |a 10.1155/2018/3272098 | ||
| 520 | |a Regenerative medicine for bone tissue mainly depends on efficient recruitment of endogenous or transplanted stem cells to guide bone regeneration. Platelet-derived growth factor (PDGF) is a functional factor that has been widely used in tissue regeneration and repair. However, the short half-life of PDGF limits its efficacy, and the mechanism by which PDGF regulates stem cell-based bone regeneration still needs to be elucidated. In this study, we established genetically modified PDGF-B-overexpressing bone marrow stromal cells (BMSCs) using a lentiviral vector and then explored the mechanism by which PDGF-BB regulates BMSC-based vascularized bone regeneration. Our results demonstrated that PDGF-BB increased osteogenic differentiation but inhibited adipogenic differentiation of BMSCs via the extracellular signal-related kinase 1/2 (ERK1/2) signaling pathway. In addition, secreted PDGF-BB significantly enhanced human umbilical vein endothelial cell (HUVEC) migration and angiogenesis via the phosphatidylinositol 3 kinase (PI3K)/AKT and ERK1/2 signaling pathways. We evaluated the effect of PDGF-B-modified BMSCs on bone regeneration using a critical-sized rat calvarial defect model. Radiography, micro-CT, and histological analyses revealed that PDGF-BB overexpression improved BMSC-mediated angiogenesis and osteogenesis during bone regeneration. These results suggest that PDGF-BB facilitates BMSC-based bone regeneration by enhancing the osteogenic and angiogenic abilities of BMSCs. | ||
| 546 | |a EN | ||
| 690 | |a Internal medicine | ||
| 690 | |a RC31-1245 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Stem Cells International, Vol 2018 (2018) | |
| 787 | 0 | |n http://dx.doi.org/10.1155/2018/3272098 | |
| 787 | 0 | |n https://doaj.org/toc/1687-966X | |
| 787 | 0 | |n https://doaj.org/toc/1687-9678 | |
| 856 | 4 | 1 | |u https://doaj.org/article/62d6f4bdeb84483c8b36dfb45ddce74b |z Connect to this object online. |